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Tumor necrosis factor-α promoter polymorphisms in Mexican patients with systemic lupus erythematosus (SLE)

Genes & Immunity volume 2, pages 363–366 (2001)Cite this article

Abstract

The major histocompatibility (MHC) genes including TNF-α, HSP70 and HLA genes have been associated with systemic lupus erythematosus (SLE) in several populations. In this study we analyze the polymorphism of TNF-α promoter in 51 Mexican Mestizo SLE patients and 55 ethnically-matched healthy controls by polymerase chain reaction methods. No statistically significant differences were observed in the TNF −308 allele and genotype distribution between patients and healthy controls. However, we found a significant increase in the TNF G/A −238 genotype and in the TNFA −238 allele frequencies in the SLE group when compared with healthy controls (Pc = 0.03, OR = 4.77 and Pc = 0.02, OR = 3.62, respectively). DRB1 analysis showed a similar distribution in patients and controls. Linkage disequilibrium was observed for five haplotypes: DRB1*1401-TNFA−238 (D = 0.84; D′ = 1.0; P = 0.015); DRB1*0301-TNFA−238 (D = 1.38; D′ = 0.41; P = 0.042); DRB1*1106-TNF2−308 (D = 0.9; D′ = 1.0; P = 0.0006); DRB1*1104-TNF2−308 (D = 0.83; D′ = 0.45; P = 0.02) and DRB1*1406-TNF2−308 (D = 0.83; D′ = 0.45; P = 0.02). Our data suggest that the association between the TNF-α −238 polymorphism and SLE could play a major role in disease susceptibility.

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Authors and Affiliations

  1. Molecular Biology and Immunogenetics Laboratory, Infectious Diseases Unit, Instituto Nacional de Enfermedades Respiratorias, Mexico City, Mexico

    J Zúñiga

  2. Physiology Department, Cellular Biology Section, Instituto Nacional de Cardiología Ignacio Chávez, Mexico City, Mexico

    G Vargas-Alarcón & G Hernández-Pacheco

  3. Immunology and Rheumatology Department, Instituto Nacional de la Nutrición Salvador Zubirán, Mexico City, Mexico

    C Portal-Celhay, JK Yamamoto-Furusho & J Granados

Authors
  1. J Zúñiga
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  2. G Vargas-Alarcón
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  3. G Hernández-Pacheco
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  4. C Portal-Celhay
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  5. JK Yamamoto-Furusho
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  6. J Granados
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Correspondence to J Granados.

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Zúñiga, J., Vargas-Alarcón, G., Hernández-Pacheco, G. et al. Tumor necrosis factor-α promoter polymorphisms in Mexican patients with systemic lupus erythematosus (SLE) . Genes Immun 2, 363–366 (2001). https://doi.org/10.1038/sj.gene.6363793

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