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Elements regulating angiogenesis and correlative microvessel density in benign hyperplastic and malignant prostate tissue

Prostate Cancer and Prostatic Diseases volume 6, pages 131–137 (2003)Cite this article

Abstract

Purpose: To quantify the ex vivo production of proangiogenic proteins (vascular endothelial growth factor (VEGF), basic fibroblast growth factor (bFGF), urokinase-type plasminogen activator (u-PA), tissue-type plasminogen activator (tPA)) and angiogenesis inhibitors (plasminogen activator inhibitor type-1 (PAI-1) and angiostatin) from epithelial and stromal components of primary prostate cancer (CaP) and benign prostatic hyperplasia (BPH) cultures. To perform microvessel density (MVD) counts on sections of BPH and CaP from the same prostatectomy specimens.

Scope: Angiogenic cytokine expression was measured by immunoassays and in vitro angiostatin generating capacities assessed using immunoblotting. CaP and BPH tissue was immunostained using factor VIII antibody to determine MVD.

Conclusions: Elements regulating angiogenesis are present in both primary cultures of CaP and BPH, suggesting that angiogenic ability is well established in the absence of carcinoma.

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Acknowledgements

The authors appreciate the assistance of Dr Donna Peehl, Stanford University, in the technique of establishing primary prostate cultures. CPE was supported by University of California, Davis Health System Research Award, American Cancer Society Institutional Research Award #205, The New York Academy of Medicine Edwin Beer Fellowship.

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Authors and Affiliations

  1. Department of Urology, University of California Davis School of Medicine, Sacramento, California, USA

    S-J Shih, M A Dall'Era, J Yang & C P Evans

  2. Department of Pathology, University Medical Center Nijmegen, Nijmegen, The Netherlands

    J R Westphal

  3. Department of Chemical Endocrinology, University Medical Center Nijmegen, Nijmegen, The Netherlands

    C G J Sweep

  4. Department of Pathology, University of California Davis School of Medicine, Sacramento, California, USA

    R Gandour-Edwards

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  1. S-J Shih
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Correspondence to C P Evans.

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Shih, SJ., Dall'Era, M., Westphal, J. et al. Elements regulating angiogenesis and correlative microvessel density in benign hyperplastic and malignant prostate tissue. Prostate Cancer Prostatic Dis 6, 131–137 (2003). https://doi.org/10.1038/sj.pcan.4500637

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