Skip to main content

Thank you for visiting nature.com. You are using a browser version with limited support for CSS. To obtain the best experience, we recommend you use a more up to date browser (or turn off compatibility mode in Internet Explorer). In the meantime, to ensure continued support, we are displaying the site without styles and JavaScript.

  • Techniques and Technology
  • Published:

Using GFP to study virus invasion and spread in plant tissues

Nature volume 388, pages 401–402 (1997)Cite this article

Abstract

GFP is beginning to revolutionize the study of virus movement in plants. Insertion of the gfpgene into the viral genome allows the virus to be tracked both in whole plants and also in single cells.

This is a preview of subscription content, access via your institution

Access options

Buy this article

  • Purchase on Springer Link
  • Instant access to full article PDF
Buy now

Prices may be subject to local taxes which are calculated during checkout

Figure 1
Figure 2: Leaf trichome cells under the confocal laser scanning microscope (CLSM).
Figure 3: A dual-labelled leaf in which Texas Red dextran was taken up into the veins through the cut petiole (red).
Figure 4: A single infected epidermal cell of N.enthamiana containing the PVX vector but lacking the coat protein (CP).
Figure 5: Localization of the viral movement protein (MP) of cucumber mosaic virus (CMV) expressed as a fusion to GFT.
Figure 6: Virus-mediated delivery of GFP to the endoplasmic reticulum of leaf mesophyll cells.

References

  1. Dolja, V. V. et al. Proc. Natl Acad. Sci. USA 89, 10208–10212 (1992).

    Google Scholar 

  2. Prasher, D. C. TIG 11, 320–323 (1995).

    Google Scholar 

  3. Baulcombe, D. C. et al. Plant J. 7, 1045–1053 (1995).

    Google Scholar 

  4. Santa Cruz, S. et al. Proc. Natl Acad. Sci. USA, 93, 6286–6290 (1996).

    Google Scholar 

  5. Oparka, K.J. et al.1, 412- 418 (1996).

  6. Oparka, K. J. et al. Protoplasma 189, 133–141 (1995).

    Google Scholar 

  7. Epel, B.L. et al. 173, 75- 79 (1996).

  8. Padgett, H. S. et al. Plant J. 10, 1079–1088 (1996).

    Google Scholar 

  9. Heinlein, M. et al. Science 270, 1983–1985 (1995).

    Google Scholar 

  10. Oparka, K.J. et al. (in the press).

  11. Citovsky, V. et al. The Plant Cell 4, 397–411 (1992).

    Google Scholar 

  12. Maule, A.J. Crit. Rev. Plant Sci. 9, 457-473 (1991).

  13. Deom, C.M. et al.69, 221- 224 (1992).

  14. Chalfie, M. et al. Science 263, 802–805 (1994).

    Google Scholar 

  15. Roberts, A.G. et al. (in the press).

  16. Boevink, P. et al. Plant J. 10, 935–941 (1996).

    Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Unit of Cell Biology, Scottish Crop Research Institute, Invergowrie, Dundee, DD2 5DA, UK

    Karl J. Oparka, Alison G. Roberts, Simon Santa Cruz, Petra Boevink, Denton A.M. Prior & Anna Smallcombe

  2. Bio-Rad Microscopy Division, Maylands Avenue, HP2 7TD, Hemel Hempstead, UK

    Karl J. Oparka, Alison G. Roberts, Simon Santa Cruz, Petra Boevink, Denton A.M. Prior & Anna Smallcombe

Authors
  1. Karl J. Oparka
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Alison G. Roberts
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Simon Santa Cruz
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Petra Boevink
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. Denton A.M. Prior
    View author publications

    You can also search for this author in PubMed Google Scholar

  6. Anna Smallcombe
    View author publications

    You can also search for this author in PubMed Google Scholar

Rights and permissions

Reprints and permissions

About this article

Cite this article

Oparka, K., Roberts, A., Cruz, S. et al. Using GFP to study virus invasion and spread in plant tissues. Nature 388, 401–402 (1997). https://doi.org/10.1038/41145

Download citation

  • Issue Date:

  • DOI: https://doi.org/10.1038/41145

This article is cited by

Search

Advanced search

Quick links

Nature Briefing

Sign up for the Nature Briefing newsletter — what matters in science, free to your inbox daily.

Get the most important science stories of the day, free in your inbox. Sign up for Nature Briefing