Skip to main content

Thank you for visiting nature.com. You are using a browser version with limited support for CSS. To obtain the best experience, we recommend you use a more up to date browser (or turn off compatibility mode in Internet Explorer). In the meantime, to ensure continued support, we are displaying the site without styles and JavaScript.

  • Paper
  • Published:

Development of an organ culture experimental system to investigate collagen synthesis in corpora cavernosa

International Journal of Impotence Research volume 10, pages 77–81 (1998)Cite this article

Abstract

An organ culture model of rabbit corpus cavernosum has been developed to investigate fibrillar collagen synthesis in intact organ. Rabbit peni were removed en bloc, the corpora cavernosa were dissected, sliced into 5 mm pieces and incubated in culture media up to 24 h. Tissue viability and metabolic and functional integrity were assessed by (a) lactate dehydrogenase (LDH) release, (b) protein synthesis, and (c) ability to respond to exogenously added cytokines.

Explants, maintained for 24 h in organ culture, exhibited minimal LDH release and maintained functional integrity determined from protein synthesis and ability to synthesize collagen in response to TGF-β1. These data suggest that explants of rabbit corpus cavernosum in organ culture represent a viable model to investigate connective tissue protein biosynthesis in vitro.

This is a preview of subscription content, access via your institution

Access options

Buy this article

  • Purchase on Springer Link
  • Instant access to full article PDF
Buy now

Prices may be subject to local taxes which are calculated during checkout

Similar content being viewed by others

Author information

Authors and Affiliations

  1. Department of Urology, Boston University School of Medicine, Boston, 02118, MA, USA

    PM Newhall, A Traish & RB Moreland

  2. Department of Biochemistry, Boston University School of Medicine, Boston, 02118, MA, USA

    A Traish

Authors
  1. PM Newhall
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. A Traish
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. RB Moreland
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to RB Moreland.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Newhall, P., Traish, A. & Moreland, R. Development of an organ culture experimental system to investigate collagen synthesis in corpora cavernosa. Int J Impot Res 10, 77–81 (1998). https://doi.org/10.1038/sj.ijir.3900309

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1038/sj.ijir.3900309

Keywords

Search

Advanced search

Quick links