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The crystal structure of the complex of blood coagulation factor VIIa with soluble tissue factor

Nature volume 380, pages 41–46 (1996)Cite this article

Abstract

Blood coagulation is initiated when tissue factor binds to coagulation factor Vila to give an enzymatically active complex which then activates factors IX and X, leading to thrombin generation and clot formation. We have determined the crystal structure at 2.0-Å resolution of active-site-inhibited factor VIIa complexed with the cleaved extracellular domain of tissue factor. In the complex, factor VIIa adopts an extended conformation. This structure provides a basis for understanding many molecular aspects of the initiation of coagulation.

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Authors and Affiliations

  1. Pharma Division, F. Hoffmann-La Roche, Grenzacherstrasse 124, 4002, Basle, Switzerland

    David W. Banner, Allan D'Arcy, Christiana Chène, Fritz K. Winkler & Daniel Kirchhofer

  2. Department of Medicine, Mount Sinai School of Medicine, New York, New York, 10029, USA

    Arabinda Guha & Yale Nemerson

  3. Department of Biochemistry, Mount Sinai School of Medicine, New York, New York, 10029, USA

    Yale Nemerson

  4. Department of Molecular Biophysics and Biochemistry, Yale University, New Haven, Connecticutt, 06510, USA

    William H. Konigsberg

Authors
  1. David W. Banner
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  2. Allan D'Arcy
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  3. Christiana Chène
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  4. Fritz K. Winkler
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  5. Arabinda Guha
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  6. William H. Konigsberg
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  7. Yale Nemerson
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  8. Daniel Kirchhofer
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Banner, D., D'Arcy, A., Chène, C. et al. The crystal structure of the complex of blood coagulation factor VIIa with soluble tissue factor. Nature 380, 41–46 (1996). https://doi.org/10.1038/380041a0

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