Abstract
THE RNA polymerase II carboxy-terminal domain (CTD) consists of tandem repeats of the sequence Tyr-Ser-Pro-Thr-Ser-Pro-Ser1–3. The CTD may participate in activated transcription through interaction with a high-molecular-weight mediator complex4–6. Such a role would be consistent with observations that some genes are preferentially sensitive to CTD mutations7,8. Here we investigate the function of the mouse RNA polymerase CTD in enhancer-driven transcription. Transcription by a-amanitin-resistant CTD-deletion mutants was tested by transient transfection of tissue culture cells in the presence of α-amanitin in order to inhibit endogenous RNA polymerase II. Removal of most of the CTD abolishes transcriptional activation by all enhancers tested, whereas transcription from promoters driven by Spl, a factor that typically activates housekeeping genes from positions proximal to the initiation sites, is not affected. These findings show that the CTD is essential in mediating 'enhancer'-type activation of mammalian transcription.
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References
Corden, J. L., Cadena, D. L., Ahearn, J. Jr & Dahmus, M. E. Proc. natn. Acad. Sci. U.S.A. 82, 7934–7938 (1985).
Allison, L. A., Moyle, M., Shales, M. & Ingles, C. J. Cell 42, 599–610 (1985).
Corden, J. L. & Ingles, C. J., in Transcriptional Regulation (eds McKnight, S. L. & Yamamoto, K. R.) 81–108 (Cold Spring Harbor Laboratory Press, Plainview, New York, 1992).
Thompson, C. M., Koleske, A. J., Chao, D. M. & Young, R. A. Cell 73, 1361–1375 (1993).
Kim, Y. J., Bjorklund, S., Li, Y., Sayre, M. H. & Kornberg, R. D. Cell 77, 599–608 (1994).
Koleske, A. J. & Young, R. A. Nature 368, 466–469 (1994).
Allison, L. A. & Ingles, C. J. Proc. natn. Acad. Sci. U.S.A. 86, 2794–2798 (1989).
Scafe, C. et al. Nature 347, 491–494 (1990).
Bartolomei, M. S. & Corden, J. L. Molec. cell Biol. 7, 586–594 (1987).
Müller-Storm, H. P., Sogo, J. M. & Schaffner, W. Cell 58, 767–777 (1989).
Zehring, W. A. & Greenleaf, A. L. J. biol. Chem. 265, 8351–8353 (1990).
Buratowski, S. & Sharp, P. A. Molec. cell. Biol. 10, 5562–5564 (1990).
Bartolomei, M. S., Halden, N. F., Cullen, C. R. & Corden, J. L. Molec. cell. Biol. 8, 330–339 (1988).
Gerber, H. P., Georgiev, O., Harshman, K. & Schaffner, W. Nucleic Acids Res. 20, 5855–5856 (1992).
Gill, G., Pascal, E., Tseng, Z. H. & Tjian, R. Proc. natn. Acad. Sci. U.S.A. 91, 192–196 (1994).
Guarente, L. Cell 36, 799–800 (1984).
Banerji, J., Rusconi, S. & Schaffner, W. Cell 27, 299–308 (1981).
Moreau, P. et al. Nucleic Acids Res. 9, 6047–6068 (1981).
Seipel, K., Georgiev, O. & Schaffner, W. EMBO J. 11, 4961–4968 (1992).
Rusconi, S., Severne, Y., Georgiev, O., Galli, I. & Wieland, S. Gene 89, 211–221 (1990).
Towbin, H., Staehelin, T. & Gordon, J. Proc. natn. Acad. Sci. U.S.A. 76, 4350–4354 (1979).
Westin, G., Gerster, T., Mueller, M. M., Schaffner, G. & Schaffner, W. Nucleic Acids Res. 15, 6787–6798 (1987).
Radtke, F. et al. EMBO J. 12, 1355–1362 (1993).
Schatt, M. D., Rusconi, S. & Schaffner, W. EMBO J. 9, 481–487 (1990).
Wieland, S., Gail, I., Schatt, M., Severne, Y. & Rusconi, S. in Activation of Hormone and Growth Factor Receptors (eds Sekeris, C. E. & Alexis, M.) 215–225 (Kluwer Academic, Dordrecht, 1990).
Seipel, K., Georgiev, O. & Schaffner, W. EMBO J. 11, 4961–4968 (1992).
Severne, Y., Wieland, S., Schaffner, W. & Rusconi, S. EMBO J. 7, 2503–2508 (1988).
Banerji, J., Olson, L. & Schaffner, W. Cell 33, 729–740 (1983).
Muller M. M., Ruppert, S., Schaffner, W. & Mathias, P. Nature 336, 544–551 (1988).
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Gerber, HP., Hagmann, M., Seipel, K. et al. RNA polymerase II C-terminal domain required for enhancer-driven transcription. Nature 374, 660–662 (1995). https://doi.org/10.1038/374660a0
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DOI: https://doi.org/10.1038/374660a0
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