Abstract
THE concentration of Dorsal protein in the nucleus determines cell fate along the dorsoventral axis of the Drosophila embryo1–13. The dorsal-group genes and the cactus gene are required for production and transmission of a localized signal on the ventral side of the embryo4,5 which determines the position of the highest nuclear concentration of Dorsal protein1–3. The ventralizing signal produced in somatic cells6 is transmitted through the perivitelline space7 to the integral membrane protein Toll8. Inside the embryo it leads to dissociation of the cytoplasmic Dorsal–Cactus complex and subsequent nuclear localization of Dorsal protein9,10. Two components are known to mediate the signal transduction between Toll and Dorsal–Cactus11,12: Pelle, a serine/threonine protein kinase13, and Tube, a protein with an unknown biochemical activity14. Here we construct gain-of-function alleles of pelle and tube and show that pelle functions downstream of tube. In addition, Pelle and Tube interact directly with one another. We propose that Tube is a direct activator of the protein kinase Pelle.
This is a preview of subscription content, access via your institution
Access options
Subscribe to this journal
Receive 51 print issues and online access
$199.00 per year
only $3.90 per issue
Buy this article
- Purchase on Springer Link
- Instant access to full article PDF
Prices may be subject to local taxes which are calculated during checkout
Similar content being viewed by others
References
Roth, S., Stein, D. & Nüsslein-Volhard, C. Cell 59, 1189–1202 (1989).
Steward, R. Cell 59, 1179–1188 (1989).
Rushlow, C. A., Han, K., Manley, J. L. & Levine, M. Cell 59, 1165–1177 (1989).
Anderson, K. V., Jürgens, G. & Nüsslein-Volhard, C. Cell 42, 779–789 (1985).
Anderson, K. V., Bokla, L. & Nüsslein-Volhard, C. Cell 42, 791–798 (1985).
Stein, D., Roth, S., Vogelsang, E. & Nüsslein-Volhard, C. Cell 65, 725–735 (1991).
Stein, D. & Nüsslein-Volhard, C. Cell 68, 429–440 (1992).
Hashimoto, C., Hudson, K. L. & Anderson, K. V. Cell 52, 269–279 (1988).
Whalen, A. & Steward, R. J. Cell Biol. 123, 523–534 (1993).
Hagenmeier, S. Diplomarbeit, Univ. Tübingen (1992).
Roth, S., Hiromi, Y., Godt, G. & Nüsslein-Volhard, C. Development 112, 371–388 (1991).
Hecht, P. & Anderson, K. V. Genetics 135, 405–417 (1993).
Shelton, C. A. & Wasserman, S. A. Cell 72, 515–525 (1993).
Letsou, A., Alexander, S., Orth, K. & Wasserman, S. A. Proc. natn. Acad. Sci. U.S.A. 88, 810–814 (1991).
Klingler, M., Erdélyi, M., Szabad, J. & Nüsslein-Volhard, C. Nature 335, 275–277 (1988).
Sprenger, F., Stevens, L. M. & Nüsslein-Volhard, C. Nature 338, 478–483 (1989).
Dickson, B., Sprenger, F., Morrison, D. & Hafen, E. Nature 360, 600–603 (1992).
Fields, S. & Song, O.-K. Nature 340, 245–246 (1989).
Geisler, R., Bergmann, A., Hiromi, Y. & Nüsslein-Volhard, C. Cell 71, 613–621 (1992).
Kidd, S. Cell 71, 623–635 (1992).
Isoda, K. & Nüsslein-Volhard, C. Proc. natn. Acad. Sci. U.S.A. 91, 5350–5354 (1994).
Ullrich, A. & Schlessinger, J. Cell 61, 203–212 (1990).
Steward, R. Science 238, 692–694 (1987).
Gay, N. J. & Keith, F. J. Nature 351, 355–356 (1991).
Wieschaus, E. & Nüsslein-Volhard, C. in Drosophila, A Practical Approach (ed. D. B. Roberts) 199–227 (IRL, Oxford, 1986).
Sprenger, F. & Nüsslein-Volhard, C. Cell 71, 987–1001 (1992).
Nishida, Y. et al. EMBO J. 7, 775–781 (1988).
Marcey, D., Watkins, W. S. & Hazelrigg, T. EMBO J. 10, 4259–4266 (1991).
Gyuris, J., Golemis, E., Chertkov, H. & Brent, R. Cell 75, 791–803 (1993).
Morrison, D. K. et al. Cell 58, 649–657 (1989).
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Großhans, J., Bergmann, A., Haffter, P. et al. Activation of the kinase Pelle by Tube in the dorsoventral signal transduction pathway of Drosophila embryo. Nature 372, 563–566 (1994). https://doi.org/10.1038/372563a0
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1038/372563a0
This article is cited by
-
A non-canonical Raf function is required for dorsal–ventral patterning during Drosophila embryogenesis
Scientific Reports (2022)
-
Influence of Metarhizium anisopliae (IMI330189) and Mad1 protein on enzymatic activities and Toll-related genes of migratory locust
Environmental Science and Pollution Research (2019)
-
Peli: a family of signal-responsive E3 ubiquitin ligases mediating TLR signaling and T-cell tolerance
Cellular & Molecular Immunology (2012)
-
The Drosophila systemic immune response: sensing and signalling during bacterial and fungal infections
Nature Reviews Immunology (2007)
-
Nuclear factor-kappa B pathways in Drosophila
Oncogene (2006)
Comments
By submitting a comment you agree to abide by our Terms and Community Guidelines. If you find something abusive or that does not comply with our terms or guidelines please flag it as inappropriate.
