Abstract
SEVERAL bacteriophages use the Escherichia coli GroES and GroEL chaperonins for folding and assembly of their morpho-genetic structures1. Bacteriophage T4 is unusual in that it encodes a specialized protein (Gp31) that is thought to interact with the host GroEL and to be absolutely required for the correct assembly of the major capsid protein (Gp23) in vivo2–4. Here we show that despite the absence of amino-acid sequence similarity between Gp31 and GroES5,6, Gp31 can functionally substitute for the GroES co-chaperonin in the morphogenesis of bacteriophages Λ and T5, the in vivo and in vitro chaperonin-dependent assembly of ribulose bisphosphate carboxylase (Rubisco), as well as overall bacterial growth at the non-permissive temperature. Like GroES, the bacteriophage Gp31 protein forms a stable complex with the E. coli GroEL protein in the presence of Mg-ATP and inhibits the ATPase activity of GroEL in vitro
This is a preview of subscription content, access via your institution
Access options
Subscribe to this journal
Receive 51 print issues and online access
$199.00 per year
only $3.90 per issue
Buy this article
- Purchase on Springer Link
- Instant access to full article PDF
Prices may be subject to local taxes which are calculated during checkout
Similar content being viewed by others
References
Zeilstra-Ryalls, J., Fayet, O. & Georgopoulos, C. A. Rev. Microbiol. 45, 301–325 (1991).
Laemmli, U. K., Geguin, F. & Gujer-Kellenberger, G. J. molec. Biol. 47, 69–85 (1970).
Georgopoulos, C. P. et al. Nature New Biol. 239, 38–41 (1972).
Takano, T. & Kakefuda, T. Nature New Biol. 239, 34–37 (1972).
Keppel, F. et al. Gene 86, 19–25 (1990).
Nivinskas, R. & Black, L. W. Gene 73, 251–257 (1988).
Landry, S. J. et al. Nature 364, 255–258 (1993).
Fayet, O., Louarn, J.-M. & Georgopoulos, C. Molec. gen. Genet. 202, 435–445 (1986).
Viitanen, P. V., Gatenby, A. A. & Lorimer, G. H. Protein Sci. 1, 363–369 (1992).
Horwich, A. L. et al. Cell 74, 909–917 (1993).
Goloubinoff, P., Gatenby, A. A. & Lorimer, G. H. Nature 337, 44–47 (1989).
Viitanen, P. V. et al. Biochemistry 29, 5665–5671 (1990).
Jackson, G. S. et al. Biochemistry 32, 2554–2563 (1993).
Todd, M. J., Viitanen, P. V. & Lorimer, G. H. Biochemistry 32, 8560–8567 (1993).
Sternberg, N. J. molec. Biol. 76, 1–23 (1973).
Gatenby, A. A., van der Vies, S. M. & Bradley, D. Nature 314, 617–620 (1985).
Lorimer, G. H., Badger, M. R. & Andrews, J. T. Analyt. Biochem. 78, 66–75 (1977).
Castillo, J. C. & Black, L. W. J. biol. Chem. 253, 2132–2139 (1978).
Goloubinoff, P., Christeller, J. T., Gatenby, A. A. & Lorimer, G. H. Nature 342, 884–889 (1989).
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
van der Vies, S., Gatenby, A. & Georgopoulos, C. Bacteriophage T4 encodes a co-chaperonin that can substitute for Escherichia coli GroES in protein folding. Nature 368, 654–656 (1994). https://doi.org/10.1038/368654a0
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1038/368654a0
This article is cited by
-
Distinct Stabilities of the Structurally Homologous Heptameric Co-Chaperonins GroES and gp31
Journal of the American Society for Mass Spectrometry (2019)
-
Novel chaperonins are prevalent in the virioplankton and demonstrate links to viral biology and ecology
The ISME Journal (2017)
-
Chaperonin complex with a newly folded protein encapsulated in the folding chamber
Nature (2009)
-
Monitoring macromolecular complexes involved in the chaperonin-assisted protein folding cycle by mass spectrometry
Nature Methods (2005)
Comments
By submitting a comment you agree to abide by our Terms and Community Guidelines. If you find something abusive or that does not comply with our terms or guidelines please flag it as inappropriate.