Abstract
TRANSPORT vesicles derived from the Golgi apparatus are thought to mediate biosynthetic transport across the Golgi stack1–3. These vesicles are surrounded by a protein coat4 whose principal constituents are coatomer (a complex of seven distinct subunits or COPs)5 and ADP-ribosylation factor (ARF, an N-myristylated small GTP-binding protein). The coat proteins of the COP-coated vesicles were originally defined by ultrastructural criteria4–9, however, and it is possible that important but minor coat proteins or cytoplasmic proteins needed for coat assembly may have been overlooked. Here we show that coatomer and ARF are the only cytoplasmic proteins needed for the assembly and budding of COP-coated vesicles. COP-coated buds may therefore form essentially by self-assembly from Golgi cisternae after an initial step in which GTP is used to allow ARF binding.
This is a preview of subscription content, access via your institution
Access options
Subscribe to this journal
Receive 51 print issues and online access
$199.00 per year
only $3.90 per issue
Buy this article
- Purchase on Springer Link
- Instant access to full article PDF
Prices may be subject to local taxes which are calculated during checkout
Similar content being viewed by others
References
Orci, L., Glick, B. S. & Rothman, J. E. Cell 46, 171–184 (1986).
Melancon, P. et al. Cell 51, 1053–1062 (1987).
Orci, L., Malhotra, V., Amherdt, M., Serafini, T. & Rothman, J. E. Cell 56, 357–368 (1989).
Serafini, T. et al. Nature 349, 215–220 (1991).
Waters, M. G., Serafini, T. & Rothman, J. E. Nature 349, 248–251 (1991).
Duden, R., Griffith, G., Frank, R., Argos, P. & Kreis, T. E. Cell 64, 649–665 (1991).
Serafini, T. et al. Cell 67, 239–253 (1991).
Malhotra, V., Serafini, T., Orci, L., Shepherd, J. C. & Rothman, J. E. Cell 58, 329–336 (1989).
Schekman, R. Curr. Opin. Cell Biol. 4, 587–592 (1992).
Orci, L. et al. Nature 362, 648–652 (1993).
Rothman, J. E. & Orci, L. Nature 355, 409–415 (1992).
Caspar, D. L. D. & Klug, A. Cold Spring Harbor Symp. quant. Biol. 27, 1–24 (1962).
Schmid, S. L. BioEssays 14, 589–596 (1992).
Moore, M. S., Mahaffey, D. T., Brodsky, F. M. & Anderson, R. G. W. Science 236, 558–563 (1987).
Mahaffey, D. T., Moore, M. S., Brodsky, F. M. & Anderson, R. G. W. J. Cell Biol. 108, 1615–1624 (1989).
Mahaffey, D. T., Peeler, J. S., Brodsky, F. M. & Anderson, R. G. W. J. biol. Chem. 265, 16514–16520 (1990).
Smythe, E., Carter, L. L. & Schmid, S. L. J. Cell Biol. 119, 1163–1171 (1992).
Stamnes, M. A. & Rothman, J. E. Cell 73, 999–1005 (1993).
Donaldson, J. G., Cassel, D., Kahn, R. A. & Klausner, R. D. Proc. natn. Acad. Sci. U.S.A. 89, 6408–6412 (1992).
Palmer, D. J., Helms, J. B., Beckers, C. J. M., Orci, L. & Rothman, J. E. J. biol. Chem. 268, 12083–12089 (1993).
Kahn, R. A. et al. J. biol. Chem. 267, 13039–13046 (1992).
Waters, M. G., Beckers, C. J. M. & Rothman, J. E. Meth. Enzym. 219, 331–337 (1992).
Weiss, O., Holden, J., Rulka, C. & Kahn, R. A. J. biol. Chem. 264, 21066–21072 (1989).
Duronio, R. J. et al. Proc. natn. Acad. Sci. U.S.A. 87, 1506–1510 (1990).
Tokuyasu, K. T. Histochem. J. 12, 381–403 (1980).
Roth, J., Bendayan, M. & Orci, L. J. Histochem. Cytochem. 26, 1074–1081 (1978).
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Orci, L., Palmer, D., Amherdt, M. et al. Coated vesicle assembly in the Golgi requires only coatomer and ARF proteins from the cytosol. Nature 364, 732–734 (1993). https://doi.org/10.1038/364732a0
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1038/364732a0
This article is cited by
-
Structural and mechanistic insights into regulation of the retromer coat by TBC1d5
Nature Communications (2016)
-
ArfGAP1 generates an Arf1 gradient on continuous lipid membranes displaying flat and curved regions
The EMBO Journal (2010)
-
The evolving understanding of COPI vesicle formation
Nature Reviews Molecular Cell Biology (2009)
-
A role for phosphatidic acid in COPI vesicle fission yields insights into Golgi maintenance
Nature Cell Biology (2008)
-
Membrane curvature and mechanisms of dynamic cell membrane remodelling
Nature (2005)
Comments
By submitting a comment you agree to abide by our Terms and Community Guidelines. If you find something abusive or that does not comply with our terms or guidelines please flag it as inappropriate.