Abstract
THE RAD10 gene of Saccharomyces cerevisiae is required for the incision step of excision repair of ultraviolet-damaged DNA (refs 1, 2), and it functions in mitotic recombination3. RAD10 has homology to the human excision repair gene ERCC-1 (ref.4). Here we describe the purification of the protein encoded by RAD10 and show that it is a DNA-binding protein with a strong preference for single-stranded DNA. We also show that RAD1O promotes the renaturation of complementary DNA strands.
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Sung, P., Prakash, L. & Prakash, S. Renaturation of DNA catalysed by yeast DNA repair and recombination protein RAD1O. Nature 355, 743–745 (1992). https://doi.org/10.1038/355743a0
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DOI: https://doi.org/10.1038/355743a0
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