Abstract
THE CD8 glycoprotein of T cells binds nonpolymorphic regions of class I major histocompatibility complex proteins on target cells1,2 and these interactions promote antigen recognition and signalling by the T-cell receptor3–11. Studies using artificial membranes indicated that effective CD8/class I interaction is critical for response by alloantigen-specific cytotoxic T lymphocytes when class I protein is the only ligand on the antigen-bearing surface10,11. But significant CD8-mediated binding of cytotoxic T lymphocytes to non-antigenic class I protein could not be detected in the absence of the alloantigen12. These apparently contradictory findings indicate that CD8 binding to class I protein might be activated through the T-cell receptor and the results reported here demonstrate that this is the case. Treatment of cytotoxic T lymphocytes with soluble anti-T-cell receptor antibody activates adhesion of the cytotoxic T lymphocytes to class I, but not class II proteins. The specificity of this binding implies that it is mediated by CD8 and blocking by anti-CD8 antibodies confirmed this. Furthermore, binding of CD8 to class I protein resulted in generation of an additional signal(s) necessary to initiate response at low T-cell receptor occupancy levels.
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References
Norment, A. M. et al. Nature 336, 79–81 (1988).
Rosenstein, Y., Ratnofsky, S., Burakoff, S. J. & Herrmann, S. H. J. exp. Med. 169, 149–160 (1989).
Nakayama, E. et al. Proc. natn. Acad. Sci. U.S.A. 76, 1977–1981 (1979).
Swain, S. L. Proc. natn. Acad. Sci. U.S.A. 78, 7101–7105 (1981).
Biddison, W. E., Rao, A. P., Talle, M. A., Goldstein, G. & Shaw, S. J. exp. Med. 156, 1065–1076 (1982).
Meuer, S. C., Schlossmann, S. F. & Reinherz, E. L. Science 218, 471–473 (1982).
MacDonald, H. R., Glasebrook, A. L., Bron, C., Kelso, A. & Cerottini, J.-C. Immunol. Rev. 68, 89–115 (1982).
Gabert, J. et al. Cell 50, 545–554 (1987).
Dembic, Z. et al. Nature 326, 510–511 (1987).
Goldstein, S. A. N. & Mescher, M. F. J. Immun. 140, 3707–3711 (1988).
Kane, K. P., Sherman, L. A. & Mescher, M. F. J. Immun. 142, 4153–4160 (1989).
Kane, K. P., Goldstein, S. A. N. & Mescher, M. F. Eur. J. Immun. 18, 1925–1929 (1988).
Bonavida, B., Bradley, T. P. & Grimm, E. A. Immun. Today 4, 196–200 (1983).
Perez, P., Bluestone, J. A., Stephany, D. A. & Segal, D. M. J. Immun. 134, 478–485 (1985).
Blue, M.-L. et al. J. Immun. 139, 3949–3954 (1987).
Veillette, A., Bookman, M. A., Horak, E. M. & Bolen, J. B. Cell 55, 301–308 (1988).
Veillette, A., Bookman, M. A., Horak, E. M., Samelson, L. E. & Bolen, J. B. Nature 338, 257–259 (1989).
Takayama, H. et al. J. Immun. 138, 566–569 (1987).
Wadsworth, S., Yui, K. & Greene, M. I. Proc. natn. Acad. Sci. U.S.A. 86, 1018–1022 (1989).
Emmrich, F., Strittmatter, U. & Eichmann, K. Proc. natn. Acad. Sci. U.S.A. 83, 8298–8302 (1986).
Owens, T., Fazekas de St. Groth, B. F. & Miller, J. F. A. P. Proc. natn. Acad. Sci. U.S.A. 84, 9209–9213 (1986).
Emmrich, F., Kanz, L. & Eichmann, K. Eur. J. Immun. 17, 529–534 (1987).
Saizawa, K., Rojo, J. & Janeway, C. A. Nature 328, 260–263 (1987).
Janeway, C. A. Immun. Today 10, 234–238 (1989).
Detmers, P. A., Wright, S. D., Olsen, E., Kimball, B. & Cohn, Z. A. J. Cell Biol. 105, 1137–1145 (1987).
Sibley, D. R., Benovic, J. L., Caron, M. G. & Lefkowitz, R. J. Cell 48, 913–922 (1987).
Springer, T. A., Dustin, M. L., Kishimoto, T. K. & Marlin, S. D. A. Rev. Immun. 5, 223–252 (1987).
Martz, E. Hum. Immun. 18, 3–37 (1987).
Dustin, M. L. & Springer, T. A. Nature 341, 619–624 (1989).
van Kooyk, Y., van de Wiel-van Kemenade, P., Weder, P., Kuijpers, T. W. & Figdor, C. G. Nature 342, 811–813 (1989).
Kane, K. P., Champoux, P. & Mescher, M. F. Molec. Immun. 26, 759–768 (1989).
Cobbold, S. P., Jayasuriya, A., Nash, A., Prospero, T. D. & Waldmann, H. Nature 312, 548–551 (1984).
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O'Rourke, A., Rogers, J. & Mescher, M. Activated CD8 binding to class I protein mediated by the T-cell receptor results in signalling. Nature 346, 187–189 (1990). https://doi.org/10.1038/346187a0
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DOI: https://doi.org/10.1038/346187a0
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