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Induction of CD8+ cytotoxic T cells by immunization with purified HIV-1 envelope protein in ISCOMs

Nature volume 344, pages 873–875 (1990)Cite this article

Abstract

To reduce the risks of immunization with killed or live attenuated virus vaccines, it may be advantageous to use a pure, defined antigen that contains determinants for both humoral and cellular immunity. However, although most non-living intact protein pre-parations induce antibodies and CD4+major histocompatibility complex (MHC) class Il-restricted helper and/or cytotoxic T lymphocytes (CTL), they do not elicit CD8+ MHC class I restricted CTL1,2. Indeed, with a few exceptions3,4 it has not so far been possible to induce CD8+ CTL by immunizing with intact soluble proteins. We show here that a single subcutaneous immunization in mice with immunostimulating complexes containing either purified intact gploO envelope glycoprotein of the human immunodeficiency virus (HIV)-l or influenza haemagglutinin results in reproducible and long-lasting priming of HIV specific or influenza-specific CD8+, MHC class I restricted CTL.

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Author notes

  1. Hidemi Takahashi

    Present address: Department of Microbiology and Immunology, Nippon Medical School, Tokyo, 113, Japan

  2. Jay A. Berzofsky: To whom correspondence should be addressed.

Authors and Affiliations

  1. Molecular Immunogenetics and Vaccine Research Section, Metabolism Branch, National Cancer Institute, National Institutes of Health, Bethesda, Maryland, 20892, USA

    Hidemi Takahashi, Toshiyuki Takeshfta & Jay A. Berzofsky

  2. Department of Virology, National Veterinary Institute, Biomédical Center, S-751 23, Uppsala, Sweden

    Bror Morein

  3. Repligen Corporation, Cambridge, Massachusetts, 02139, USA

    Scott Putney

  4. Lymphocyte Biology Section, Laboratory of Immunology, National Institute of Allergy and Infectious Disease, National Institutes of Health, Bethesda, Maryland, 20892, USA

    Ronald N. Germain

  5. National Cancer Institute, National Institutes of Health, Building 10, Room 6B-12, Bethesda, MD, 20892, USA

    Jay A. Berzofsky

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  1. Hidemi Takahashi
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Takahashi, H., Takeshfta, T., Morein, B. et al. Induction of CD8+ cytotoxic T cells by immunization with purified HIV-1 envelope protein in ISCOMs. Nature 344, 873–875 (1990). https://doi.org/10.1038/344873a0

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