Abstract
THE molecular recognition of specific transfer RNAs by the appropriate aminoacyl-tRNA synthetase is an important step in determining the accuracy of translation of the genetic message from nucleic acids into proteins. Recent studies using variant tRNAs with specifie sequence modifications have indicated particular regions that determine their identity1–8. Here we consider whether the base modifications commonly found in tRNAs9 contribute to their identity. Although unmodified tRNAAsp is charged with aspartate as efficiently as the modified native tRNA, it is mischarged with arginine with considerably increased efficiency. Our results indicate that post-transcriptional modification of tRNAs introduces structural 'anti-determinants', restricting the efficiency with which the tRNAs are charged with inappropriate amino acids.
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References
Sampson, J. & Uhlenbeck, O. C. Proc. natn. Acad. Sci. U.S.A. 85, 1033–1037 (1988).
Hou, Y.-H. & Schimmel, P. Nature 333, 140–145 (1988).
McClain, W. H. & Foss, K. Science 240, 793–796 (1988).
Rogers, J. & Söll, D. Proc. natn. Acad. Sci. U.S.A. 85, 6627–6631 (1988).
Schulman, L. H. & Pelka, H. Science 242, 765–768 (1988).
Sampson, J. R., DiRenzo, A. B., Behlen, L. S. & Uhlenbeck, O. C. Science 243, 1363–1366 (1989).
Schimmel, P. Biochemistry 28, 2747–2759 (1989).
Normanly, J. & Abelson, J. A. Rev. Biochem. 58, 1029–1049 (1989).
Björk, G. R. et al. A. Rev. Biochem. 56, 263–287 (1987).
Moras, D. et al. Nature 288, 669–674 (1980).
Westhof, E., Dumas, P. & Moras, D. J. molec. Biol. 184, 119–145 (1985).
Romby, P. et al. J. molec. Biol. 184, 455–471 (1985).
Romby, P., Moras, D., Dumas, P., Ebel, J. P. & Giegé, R. J. molec. Biol. 195, 193–204 (1987).
Dunn, J. J. & Studier, F. W. J. molec. Biol. 166, 477–535 (1983).
Sprinzl, M., Hartmann, T., Weber, J., Blank, J. & Zeidler, R. Nucleic Acids Res. 17, 1–172 (1989).
Peattie, D. A. & Gilbert, W. Proc. natn. Acad. Sci. U.S.A. 77, 4679–4682 (1980).
Krzyzosiak, W. J. et al. Biochemistry 27, 5771–5777 (1988).
Hall, K. B., Sampson, J. R., Uhlenbeck, O. C. & Redfield, A. G. Biochemistry 28, 5794–5800 (1989).
Grosjean, H. & Kubli, E. in Microinjection and Organelle Transplantation Techniques (eds Celis, J. E., Graessman, A. & Loyter, A.) 304–326 (Academic, New York, 1986).
Giegé, R. et al. Eur. J. Biochem. 45, 351–362 (1974).
Yarus, M. Biochemistry 11, 2352–2361 (1972).
Ebel, J. P. et al. Biochemie 55, 547–557 (1973).
Roe, B., Michael, M. & Dudock, B. Nature new Biol. 246, 135–138 (1973).
Muramatsu, T. et al. Nature 336, 179–181 (1988).
Lorber, B. et al. Biochem. biophys. Res. Commun. 117, 259–267 (1983).
Gangloff, J., Schutz, A. & Dirheimer, G. Eur J. Biochem. 65, 177–182 (1976).
Gangloff, J., Keith, G., Ebel, J. P. & Dirheimer, G. Nature new Biol. 230, 125–127 (1971).
Dietrich, A., Kern, D., Bonnet, J., Giegé, R. & Ebel, J. P. Eur J. Biochem. 70, 147–158 (1976).
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Perret, V., Garcia, A., Grosjean, H. et al. Relaxation of a transfer RNA specificity by removal of modified nucleotides. Nature 344, 787–789 (1990). https://doi.org/10.1038/344787a0
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DOI: https://doi.org/10.1038/344787a0
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