Abstract
TRUE Upases attack triacylglycerols and act at an oil–water interface; they constitute a ubiquitous group of enzymes catalysing a wide variety of reactions, many with industrial potential. But so far the three-dimensional structure has not been reported for any lipase. Here we report the X-ray structure of the Mucor miehei triglyceride lipase and describe the atomic model obtained at 3.1 Å resolution and refined to 1.9 Å resolution. It reveals a Ser .. His .. Asp trypsin-like catalytic triad with an active serine buried under a short helical fragment of a long surface loop.
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Brady, L., Brzozowski, A., Derewenda, Z. et al. A serine protease triad forms the catalytic centre of a triacylglycerol lipase. Nature 343, 767–770 (1990). https://doi.org/10.1038/343767a0
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DOI: https://doi.org/10.1038/343767a0
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