Abstract
IN reconstitution studies N-ethylmaleimide, a sulphydryl alkylating reagent, inhibits both fusion of endocytic vesicles1–3 and vesicular transport in the Golgi apparatus4. We show here that the same N-ethylmaleimide-sensitive factor that catalyses the vesicle-mediated transport within Golgi stacks is also required for endocytic vesicle fusion. Thus, it is likely that a common mechanism for vesicle fusion exists for both the secretory and endocytic pathways of eukaryotic cells.
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References
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Diaz, R., Mayorga, L., Weidman, P. et al. Vesicle fusion following receptor-mediated endocytosis requires a protein active in Golgi transport. Nature 339, 398–400 (1989). https://doi.org/10.1038/339398a0
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DOI: https://doi.org/10.1038/339398a0
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