Skip to main content

Thank you for visiting nature.com. You are using a browser version with limited support for CSS. To obtain the best experience, we recommend you use a more up to date browser (or turn off compatibility mode in Internet Explorer). In the meantime, to ensure continued support, we are displaying the site without styles and JavaScript.

  • Article
  • Published:

Amplification and analysis of DNA sequences in single human sperm and diploid cells

Nature volume 335, pages 414–417 (1988)Cite this article

Abstract

The use of the polymerase chain reaction for analysing DNA sequences in individual diploid cells and human sperm shows that two genetic loci can be co-amplified from a single sperm, which may allow the analysis of previously inaccessible genetic phenomena.

This is a preview of subscription content, access via your institution

Access options

Buy this article

  • Purchase on Springer Link
  • Instant access to full article PDF
Buy now

Prices may be subject to local taxes which are calculated during checkout

Similar content being viewed by others

References

  1. Donis-Keller, H. et al. Cell 51, 319–337 (1987).

    Article  CAS  PubMed  Google Scholar 

  2. Botstein, D., White, R. L., Skolnick, M. & Davis, R. W. Am. J. hum. Genet. 32, 314–331 (1980).

    CAS  PubMed  PubMed Central  Google Scholar 

  3. Saiki, R. K. et al. Science 230, 1350–1354 (1985).

    Article  ADS  CAS  PubMed  Google Scholar 

  4. Mullis, K. B. & Faloona, F. A. Meth. Enzym. 155, 335–350 (1987).

    Article  CAS  PubMed  Google Scholar 

  5. Saiki, R. K., Bugwan, T. L., Horn, G. T., Mullis, K. B. & Erlich, H. A. Nature 324, 163–166 (1986).

    Article  ADS  CAS  PubMed  Google Scholar 

  6. Saiki, R. K. et al. Science 239, 487–491 (1988).

    Article  ADS  CAS  PubMed  Google Scholar 

  7. Conner, B. J. et al. Proc. natn. Acad. Sci. U.S.A. 80, 278–282 (1983).

    Article  ADS  CAS  Google Scholar 

  8. Lindgren, V., Lusky, K. L., Russel, D. W. & Francke, U. Proc. natn. Acad. Sci. U.S.A. 82, 8567–8571 (1985).

    Article  ADS  CAS  Google Scholar 

  9. Hobbs, H. H., Esser, V. & Russell, D. W. Nucleic Acids Res. 15, 379 (1987).

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  10. Puwaravutipanich, T. & Panyim, S. Expl. Cell Res. 90, 153–158 (1975).

    Article  CAS  Google Scholar 

  11. Scharf, S. J., Horn, G. T. & Erlich, H. A. Science 233, 1076–1078 (1986).

    Article  ADS  CAS  PubMed  Google Scholar 

  12. Renwick, J. H. Br. med. Bull. 25, 65–73 (1969).

    Article  CAS  PubMed  Google Scholar 

  13. Chakravarti, A. et al. Am. J. hum. Genet. 36, 1239–1258 (1984).

    CAS  PubMed  PubMed Central  Google Scholar 

  14. Chakravarti, A., Elbein, S. C. & Permutt, M. A. Proc. natn. Acad. Sci. U.S.A. 83, 1045–1049 (1986).

    Article  ADS  CAS  Google Scholar 

  15. Migone, N., de Lange, G., Piazza, A. & Cavalli-Sforza, L. L. Am. J. hum. Genet. 37, 1146–1163 (1985).

    CAS  PubMed  PubMed Central  Google Scholar 

  16. Gill, P., Jeffreys, A. J. & Werrett, D. J. Biotechnology 3, 1008–1012 (1985).

    Google Scholar 

Download references

Author information

Author notes

  1. Norman Arnheim: To whom correspondence should be addressed.

Authors and Affiliations

  1. Molecular Biology Section, Department of Biological Sciences, University of Southern California, Los Angeles, California, 90089-0371, USA

    Honghua Li, Xiangfeng Cui & Norman Arnheim

  2. Department of Human Genetics, Cetus Corporation, 1400 53rd Street, Emeryville, California, 94608, USA

    Ulf B. Gyllensten, Randall K. Saiki & Henry A. Erlich

Authors
  1. Honghua Li
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Ulf B. Gyllensten
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Xiangfeng Cui
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Randall K. Saiki
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. Henry A. Erlich
    View author publications

    You can also search for this author in PubMed Google Scholar

  6. Norman Arnheim
    View author publications

    You can also search for this author in PubMed Google Scholar

Rights and permissions

Reprints and permissions

About this article

Cite this article

Li, H., Gyllensten, U., Cui, X. et al. Amplification and analysis of DNA sequences in single human sperm and diploid cells. Nature 335, 414–417 (1988). https://doi.org/10.1038/335414a0

Download citation

  • Received:

  • Accepted:

  • Issue Date:

  • DOI: https://doi.org/10.1038/335414a0

This article is cited by

Comments

By submitting a comment you agree to abide by our Terms and Community Guidelines. If you find something abusive or that does not comply with our terms or guidelines please flag it as inappropriate.

Search

Advanced search

Quick links

Nature Briefing

Sign up for the Nature Briefing newsletter — what matters in science, free to your inbox daily.

Get the most important science stories of the day, free in your inbox. Sign up for Nature Briefing