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Essential role for phosphatidylinositol 4,5-bisphosphate in yeast cell proliferation

Nature volume 333, pages 188–190 (1988)Cite this article

Abstract

The responses of mammalian cells to external signals are com-monly mediated by intracellular secondary messengers, among which are the breakdown products of phosphatidylinositol 4,5-bisphosphate (PIP2): 1,2-diacylglycerol (DG) and inositol 1,4,5-triphosphate (IP3) (refs 1–7). Although phosphoinositide turnover in the yeast Saccharomyces cerevisiae has been shown to be regulated by glucose8,9 and sterol10,11, as yet no definitive function has been ascribed to yeast phosphoinositides. We have recently developed a monoclonal antibody specific for PIP2 and reported that it inhibits mitogenesis of mammalian cells stimulated by platelet-derived growth factor and bombesin12. We now report that when introduced into yeast cells by electroporation this antibody inhibits their growth. Furthermore, several yeast mutants with temperature-dependent growth defects are altered in their sensitiv-ity to our antibody and are found to have specific alterations in their phosphoinositide metabolism.

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Authors and Affiliations

  1. Institute of Applied Microbiology, University of Tokyo, Bunkyo-ku, Tokyo, 113, Japan

    Isao Uno & Tatsuo Ishikawa

  2. Department of Pharmacology, Tokyo Metropolitan Institute of Gerontology, Itabashi-ku, Tokyo, 173, Japan

    Kiyoko Fukami, Hiroyuki Kato & Tadaomi Takenawa

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  1. Isao Uno
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  5. Tatsuo Ishikawa
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Uno, I., Fukami, K., Kato, H. et al. Essential role for phosphatidylinositol 4,5-bisphosphate in yeast cell proliferation. Nature 333, 188–190 (1988). https://doi.org/10.1038/333188a0

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