Skip to main content

Thank you for visiting nature.com. You are using a browser version with limited support for CSS. To obtain the best experience, we recommend you use a more up to date browser (or turn off compatibility mode in Internet Explorer). In the meantime, to ensure continued support, we are displaying the site without styles and JavaScript.

  • Letter
  • Published:

Sequence similarity of phospholipase C with the non-catalytic region of src

Nature volume 332, pages 269–272 (1988)Cite this article

Abstract

The production of the second messenger molecules diacylglycerol and inositol 1,4,5-trisphosphate1 is mediated by activated phos-phatidylinositol-specific phospholipase C (PLC) enzymes2,3. Here we report the cloning of a bovine brain complementary DNA encoding an enzyme PLC-148 that is characterized by calcium-dependent and phosphatidylinositol-specific phospholipase C activity when expressed in mammalian cells. Bovine brain messenger RNA contains a 7.5-kilobase transcript corresponding to the isolated cDNA; a related transcript of the same size is present in mRNA from some but not all human cell lines tested. Southern blot analysis of the bovine genome indicated that one or possibly two genes hybridize to the cloned PLC-148 cDNA. There is a striking sequence similarity between specific regions of PLC-148 and the non-catalytic domain of the non-receptor tyrosine kinases. The newly characterized crk transforming gene of the avian sarcoma virus CT10 also contains extensive sequence similarities with PLC-148.

This is a preview of subscription content, access via your institution

Access options

Buy this article

  • Purchase on Springer Link
  • Instant access to full article PDF
Buy now

Prices may be subject to local taxes which are calculated during checkout

Similar content being viewed by others

References

  1. Berridge, M. J. Biochem. J. 220, 345–360 (1984).

    Article  CAS  Google Scholar 

  2. Berridge, M. J. A. Rev. Biochem. 56, 159–194 (1987).

    Article  CAS  Google Scholar 

  3. Majerus et al. Science 234, 1519–1526 (1986).

    Article  ADS  CAS  Google Scholar 

  4. Ryu, S. H., Suh, P., Cho, K. S., Lee, K. Y. & Rhee, S. G. Proc. natn. Acad. Sci. U.S.A. 84, 6649–6653 (1987).

    Article  ADS  CAS  Google Scholar 

  5. Hofmann, S. L. & Majerus, P. W. J. biol. Chem. 257, 6461–6469 (1982).

    CAS  PubMed  Google Scholar 

  6. Ryu, H. R., Cho, K. S., Suh, P. & Rhee, S. G. Biochem. biophys. Res. Commun. 141, 137–144 (1986).

    Article  CAS  Google Scholar 

  7. Sadowski, I., Stone, J. & Pawson, T. Molec. cell. Biol. 6, 4396–4408 (1986).

    Article  CAS  Google Scholar 

  8. Mayer, B. J., Hamaguchi, M. & Hanafusa, H. Nature, this issue.

  9. Jove, R. & Hanafusa, H. An. Rev. cell Biol. 3, 31–56 (1987).

    Article  CAS  Google Scholar 

  10. McCarley, D. J., Parsons, J. T., Benjamin, D. C. & Parsons, S. J. J. Virol. 61, 1927–1937 (1987).

    CAS  PubMed  PubMed Central  Google Scholar 

  11. Kozak, M. Nucleic Acids Res. 20, 8125–8148 (1987).

    Article  Google Scholar 

  12. Jacobs, K. et al. Nature 313, 806–810 (1985).

    Article  ADS  CAS  Google Scholar 

  13. Toole, J. J. et al. Nature 312, 342–347 (1984).

    Article  ADS  CAS  Google Scholar 

  14. Knopf, J. L. et al. Cell 46, 491–502 (1986).

    Article  CAS  Google Scholar 

  15. Takeya, T., Feldman, R. A. & Hanafusa, H. J. Virol. 44, 1–12 (1982).

    CAS  PubMed  PubMed Central  Google Scholar 

  16. Simon, M. A., Drees, B., Kornberg, T. & Bishop, M. J. Cell 42, 831–840 (1985).

    Article  CAS  Google Scholar 

  17. Kitamura, N. et al. Nature 297, 205–208 (1982).

    Article  ADS  CAS  Google Scholar 

  18. Marth, J. D., Peet, R., Krebs, E. G. & Perlmutter, R. M. Cell 43, 393–404 (1985).

    Article  CAS  Google Scholar 

  19. Semba, K. et al. Proc. natn. Acad. Sci. U.S.A. 83, 5459–5955 (1986).

    Article  ADS  CAS  Google Scholar 

  20. Quintrell, N. et al. Molec. cell biol. 7, 2267–2275 (1987).

    Article  CAS  Google Scholar 

  21. Yamanashi, Y. et al. Molec. cell. Biol. 7, 237–243 (1987).

    Article  CAS  Google Scholar 

  22. Naharro, G. K., Robbins, K. C. & Reddy, E. P. Science 223, 613–66 (1984).

    Article  Google Scholar 

  23. Reddy, E. P. et al. Proc. natn. Acad. Sci. U.S.A. 80, 3624–3628 (1983).

    ADS  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Department of Molecular Biology, Genetics Institute Inc., 87 Cambridge Park Drive, Cambridge, Massachusetts, 02142, USA

    Mark L. Stahl, Catherine R. Ferenz, Kerry L. Kelleher, Ron W. Kriz & John L. Knopf

Authors
  1. Mark L. Stahl
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Catherine R. Ferenz
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Kerry L. Kelleher
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Ron W. Kriz
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. John L. Knopf
    View author publications

    You can also search for this author in PubMed Google Scholar

Rights and permissions

Reprints and permissions

About this article

Cite this article

Stahl, M., Ferenz, C., Kelleher, K. et al. Sequence similarity of phospholipase C with the non-catalytic region of src. Nature 332, 269–272 (1988). https://doi.org/10.1038/332269a0

Download citation

  • Received:

  • Accepted:

  • Issue Date:

  • DOI: https://doi.org/10.1038/332269a0

This article is cited by

Comments

By submitting a comment you agree to abide by our Terms and Community Guidelines. If you find something abusive or that does not comply with our terms or guidelines please flag it as inappropriate.

Search

Advanced search

Quick links

Nature Briefing

Sign up for the Nature Briefing newsletter — what matters in science, free to your inbox daily.

Get the most important science stories of the day, free in your inbox. Sign up for Nature Briefing