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  • Cell-Based Therapy
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Cell-Based Therapy

Efficient transduction of mature CD83+ dendritic cells using recombinant adenovirus suppressed T cell stimulatory capacity

Gene Therapy volume 7, pages 249–254 (2000)Cite this article

Abstract

We have developed a culture method for the foreign serum-free generation of highly immunostimulatory, CD83+ human dendritic cells (DC). In this study, we evaluated the feasibility and consequences of endogenously expressing antigens in mature DC using adenoviral vectors. Transduction of DC with Ad-EGFP demonstrated endogenous fluorescence in 50–85% of CD83+ DC. Ad-transduced DC stimulated the proliferation of allogeneic CD8+ and CD4+ T cells at low DC: T cell ratios. However, at high DC: T cell ratios the stimulatory capacity of Ad-transduced DC was suppressed. This immunosuppressive effect was confirmed by demonstrating that the stimulatory function of untreated DC could be suppressed in a dose-dependent manner by addition of Ad-transduced DC. Furthermore, transwell experiments suggested that direct cell contact was required. Taken together, our results demonstrate the feasibility of efficiently expressing antigens in CD83+ DC using adenoviruses. However, immunosuppressive effects must be considered and carefully studied before Ad-transduced DC are employed for clinical trials.

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Acknowledgements

This work was supported by grants No. SFB-432 (to AE) and Tu90/2-1 (to TT) from the Deutsche Forschungsgemeinschaft.

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  1. H Jonuleit and T Tüting: The first two authors contributed equally

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  1. Department of Dermatology, J Gutenberg-University, Langenbeckstrasse 1, Mainz, D-55101, Germany

    H Jonuleit, T Tüting, J Steitz, J Brück, A Giesecke, K Steinbrink, J Knop & A H Enk

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  1. H Jonuleit
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Jonuleit, H., Tüting, T., Steitz, J. et al. Efficient transduction of mature CD83+ dendritic cells using recombinant adenovirus suppressed T cell stimulatory capacity. Gene Ther 7, 249–254 (2000). https://doi.org/10.1038/sj.gt.3301077

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