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  • Nonviral Transfer Technology
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Gene transfer facilitated by a cellular targeting molecule, reovirus protein σ1

Gene Therapy volume 7, pages 61–69 (2000)Cite this article

Abstract

To facilitate eventual genetic vaccination of mucosal tissues, a receptor-mediated gene transfer system was devised using the reovirus adhesin, protein σ1. Highly efficient uptake and internalization of protein σ1 polylysine (PL) DNA complexes could be demonstrated by fluorescent microscopy. Successful cellular transfection of rodent and human cell lines was obtained with the recombinant protein σ1 as a PL–DNA complex, and could be shown to be receptor-specific. Transfection efficiency was dependent upon the ratio of DNA complexed to protein σ1-PL and chloroquine treatment improved transfection efficiency dramatically. To test its ability to bind a mucosal inductive tissue, recombinant protein σ1 was specifically bound to the nasal-associated lymphoid tissues (NALT). Thus, recombinant protein σ1-PL–DNA conjugates can efficiently bind and transfect cells that express the receptor for protein σ1.

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Acknowledgements

We thank Mr Peter Hillemeyer for his assistance with flow cytometry. This work was supported by US Public Health Service Grants AI-42673, S10 RR11877, and in part by Montana Agricultural Station.

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  1. Veterinary Molecular Biology, Montana State University, Bozeman, MT 59717-3610, USA

    Y Wu, M J Boysun, K L Csencsits & D W Pascual

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Wu, Y., Boysun, M., Csencsits, K. et al. Gene transfer facilitated by a cellular targeting molecule, reovirus protein σ1. Gene Ther 7, 61–69 (2000). https://doi.org/10.1038/sj.gt.3301046

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