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Protective immunization against melanoma by gp100 DNA–HVJ-liposome vaccine

Gene Therapy volume 6, pages 1768–1773 (1999)Cite this article

Abstract

DNA-based vaccine immunization effectively induces both humoral and cell-mediated immunity to antigens and can confer protection against numerous infectious diseases as well as some cancers. Human and mouse melanomas consistently express the tumor-associated antigen interacted with the melanogenesis pathway. Gp100 is immunogenic and has been shown to induce both antibody and cytotoxic T cell (CTL) responses in humans. To explore the potential use of DNA immunization to induce melanoma-specific immune responses, we assessed HVJ-AVE liposome incorporated with plasmid DNA encoding human gp100. The gp100 DNA vaccine was used in a mouse melanoma model to assess immunity against the B16 melanoma of C57BL/6 mice. Intramuscular injection of the DNA–HVJ-AVE liposomes induced both anti-gp100 antibody and CTL responses. Gp100 DNA–HVJ-AVE liposome immunization significantly delayed tumor development in mice challenged with B16 melanoma cells. Mice immunized with gp100 DNA–HVJ-AVE liposomes survived longer compared with control mice immunized with HVJ-AVE liposome alone. These results indicate that immunization with human gp100 DNA by HVJ-AVE liposomes can induce protective immunity against melanoma in this pre-clinical mouse model. This strategy may provide an effective approach for vaccine therapy with tumor-associated antigens against human melanoma.

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Acknowledgements

This work was supported by a grant for an international joint work provided by the Ministry of Education, Science and Culture of Japan.

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Authors and Affiliations

  1. Division of Gene Therapy Science, Osaka University School of Medicine, Suita, Osaka, Japan

    W-Z Zhou, Y Kaneda & R Morishita

  2. Department of Molecular Oncology, John Wayne Cancer Institute, Santa Monica, CA, USA

    S K S Huang & D S B Hoon

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  1. W-Z Zhou
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  2. Y Kaneda
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  3. S K S Huang
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  4. R Morishita
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  5. D S B Hoon
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Zhou, WZ., Kaneda, Y., Huang, S. et al. Protective immunization against melanoma by gp100 DNA–HVJ-liposome vaccine. Gene Ther 6, 1768–1773 (1999). https://doi.org/10.1038/sj.gt.3300998

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