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Human serum-resistant retroviral vector particles from galactosyl (α1–3) galactosyl containing nonprimate cell lines

Gene Therapy volume 6, pages 1397–1405 (1999)Cite this article

Abstract

Retroviral vector particles (RVP) which are resistant to inactivation by human serum will be needed for many in vivo gene therapy applications. Murine-based producer cell lines generate RVP which are inactivated by human serum, reportedly due to the presence of the galactosyl (α1–3) galactosyl carbohydrate moiety (αGal) on these and other nonprimate producer cells and RVP. Consequently, human cells (which lack the αGal moiety) have been developed as producer cell lines for generation of human serum-resistant RVP. In this study, we report that contrary to earlier reports, the presence of the αGal moiety on producer cells and RVP does not necessarily correlate with cell killing or RVP inactivation by human serum. We show that the αGal-positive ferret brain cell line, Mpf, is an excellent basal cell line for generation of RVP which have titers and serum resistance levels equal to or greater than RVP produced in human cell lines such as HT1080. Therefore, packaging cell lines need not be limited to those of human or primate origin for production of human serum-resistant RVP.

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Acknowledgements

We thank Michele Barcia for fine technical assistance and members of the North Shore University Hospital core facilities, Craig Gawel, Colleen Millan, and Sara Danzi for DNA sequencing, and Cathy Rapelje for flow cytometry. This work was supported in part by the Boas-Marks-Spiro Gene Therapy Vector Laboratory.

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Authors and Affiliations

  1. Department of Research, Viral Vector Laboratory, North Shore University Hospital–New York University School of Medicine, Manhasset, NY, USA

    J M Mason, D E Guzowski, L O Goodwin, D Porti, K C Cronin & R G Pergolizzi

  2. Department of Laboratories, North Shore University Hospital–New York University School of Medicine, Manhasset, NY, USA

    S Teichberg

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  1. J M Mason
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  2. D E Guzowski
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  3. L O Goodwin
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Mason, J., Guzowski, D., Goodwin, L. et al. Human serum-resistant retroviral vector particles from galactosyl (α1–3) galactosyl containing nonprimate cell lines. Gene Ther 6, 1397–1405 (1999). https://doi.org/10.1038/sj.gt.3300963

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