Skip to main content

Thank you for visiting nature.com. You are using a browser version with limited support for CSS. To obtain the best experience, we recommend you use a more up to date browser (or turn off compatibility mode in Internet Explorer). In the meantime, to ensure continued support, we are displaying the site without styles and JavaScript.

  • Brief Communication
  • Published:

Rapid identification of viable retrovirus-transduced cells using the green fluorescent protein as a marker

Gene Therapy volume 4, pages 1256–1260 (1997)Cite this article

Abstract

Various methods for determining the expression of the β-galactosidase (β-gal) gene after retroviral transduction were compared as a means to assess retroviral titre. To allow better comparison, different retroviral vectors were constructed carrying two mutants of the green fluorescent protein and assessed as sensitive markers of retroviral gene transfer. It could be demonstrated that GFP is gener-ally superior to β-gal in terms of sensitivity, speed and noninvasiveness of assay, allowing easy direct FACS sorting of populations of transduced cells. This opens the possibility of enrichment by sorting of ex vivo transduced cells in gene therapy protocols.

This is a preview of subscription content, access via your institution

Access options

Buy this article

  • Purchase on Springer Link
  • Instant access to full article PDF
Buy now

Prices may be subject to local taxes which are calculated during checkout

Similar content being viewed by others

Author information

Authors and Affiliations

  1. Institute of Virology, University of Veterinary Sciences, Vienna, Austria

    D Klein & WH Günzburg

  2. Bavarian Nordic Research Institute, Munich, Germany

    D Klein, K von Rombs & B Salmons

  3. IST-Biotechnology Section, Padua, Italy

    S Indraccolo & A Amadori

  4. Department of Oncology and Surgical Sciences, University of Padua, Padua, Italy

    S Indraccolo & A Amadori

  5. Institute of Molecular Virology, GSF-National Research Center for Environment and Health, Oberschleissheim, Germany

    WH Günzburg

Authors
  1. D Klein
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. S Indraccolo
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. K von Rombs
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. A Amadori
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. B Salmons
    View author publications

    You can also search for this author in PubMed Google Scholar

  6. WH Günzburg
    View author publications

    You can also search for this author in PubMed Google Scholar

Rights and permissions

Reprints and permissions

About this article

Cite this article

Klein, D., Indraccolo, S., von Rombs, K. et al. Rapid identification of viable retrovirus-transduced cells using the green fluorescent protein as a marker. Gene Ther 4, 1256–1260 (1997). https://doi.org/10.1038/sj.gt.3300519

Download citation

  • Received:

  • Accepted:

  • Issue Date:

  • DOI: https://doi.org/10.1038/sj.gt.3300519

Keywords

This article is cited by

Search

Advanced search

Quick links