Abstract
Human immunodeficiency virus (HIV) strains can be separated into two types: HIV and HIV-related West African viruses. Site-directed serology using synthetic peptides offers possibilities for the determination of type-specific antibodies. A 22-amino-acid peptide with the sequence Ala-Ile-Glu-Lys-Tyr-Leu-Glu-Asp-Gln-Ala-Gln-Leu-Asn-Ala-Trp-Cys-Ala-Phe-Arg-Gln-Val-Cys representing a conserved region of the transmembranous protein of simian T-cell lymphotropic virus-type III (STLV-III; related to West African HIV) was used as antigen in an enzyme-linked immunosorbent assay (ELISA). In parallel, tests were performed with a pair of previously described peptides1,2, including the homologous region of the glycoprotein (gp) 41 of the HIV strain HTLV-IIIB. In tests with three groups of 20 sera it was shown that the different peptide ELISAs allowed a categorical distinction of antibodies to the two types of HIV. Tests using peptide antigens may provide excellent opportunities for large-scale testing for type-specific antibodies against HIV. The tests are simple, sensitive and specific and are readily standardized.
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References
Smith, R. S. et al. J. clin. Microbiol. 25, 1498–1504 (1987).
Rosen J. et al. in Modern Approaches to Vaccines (eds Chanock, R., Ginsberg, H., Lerner, R. & Brown, F.) (Cold Spring Harbor Laboratory Press, New York).
Barré-Sinoussi, F. et al. Science 220, 868–871 (1983).
Popovic, M., Sarngadharan, M. G., Read, E. & Gallo, R. C. Science 224, 497–500 (1984).
Abb, J. AIDS Res. 2, 93–97 (1986).
Resnick, H. W. et al. Lancet ii, 483–486 (1986).
Sarngadharan, M. G. et al. Science 224, 506–508 (1984).
Chiodi, F. et al. AIDS Res. hum. Retroviruses 3, 165–176 (1987).
Lerner, R. A. Adv. Immunol. 36, 1–44 (1984).
Wang, J. J. G., Steel, S., Wisniewolski, R. & Wang C.Y. Proc. natn. Acad. Sci. U.S.A. 83, 6159–6163 (1986).
Gnann, J. W., Schwimmbeck, P. L., Nelson, J. A., Truax, B. & Oldstone, M. B. A. J. inf. Dis. 156, 261–267 (1987).
Chiodi, F. et al. J. med. Virol. 23 (in the press).
Kanki, P. J. et al. Science 232, 238–243 (1986).
Clavel, F. et al. Science 233, 343–346 (1986).
Albert, J. et al. AIDS Res. hum. Retroviruses 3, 3–10 (1987).
Kornfeld, H., Riedel, N., Viglianti, G. A., Hirsch, V. & Mullins J. J. Nature 326, 610–613 (1987).
Franchini, G. et al. Nature 328, 539–543 (1987).
Biberfeld, G. et al. Lancet ii, 1330–1331 (1986).
Gaines, H. et al. Lancet i, 1249–1253 (1987).
Montagnier, L. Virology 144, 283–289 (1985).
Quinn, T. C., Mann, J. M., Curran, J. W. & Piot, P. Science 234, 955–961 (1986).
Kanki, P. J. et al. Science 236, 827–831 (1987).
Ratner, L. et al. Nature 313, 277–283 (1985).
Guyader, M. et al. Nature 326, 662–669 (1987).
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Norrby, E., Biberfeld, G., Chiodi, F. et al. Discrimination between antibodies to HIV and to related retroviruses using site-directed serology. Nature 329, 248–250 (1987). https://doi.org/10.1038/329248a0
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DOI: https://doi.org/10.1038/329248a0
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