Abstract
Human platelet-derived growth factor (PDGF) is a potent mitogenic polypeptide1 which is believed to be a heterodimer of A- and B-chains stabilized by interchain disulphide bonds2,3. The B-chain of PDGF is encoded by the c-sis gene, the normal cellular homologue of the transforming gene of the simian sarcoma virus (SSV)4–6. cDNA clones of the B-chain from both normal and transformed cells have mutually consistent DNA sequences7,8. Recently, an A-chain cDNA clone (D-l) was isolated from a transformed human glial cell cDNA library9. We report the complete sequence of an A-chain cDNA clone (BT-1) isolated from a normal human umbilical vein endothelial (HUVE) cell cDNA library. BT-1 differs from the sequence of the D-l clone by a 69 base pair deletion containing the predicted carboxy terminus of the protein. The mRNA levels of the A- and B-chains of PDGF in HUVE cells were analysed and shown to respond differently to the endothelial cell growth factor (ECGF).
This is a preview of subscription content, access via your institution
Access options
Subscribe to this journal
Receive 51 print issues and online access
$199.00 per year
only $3.90 per issue
Buy this article
- Purchase on Springer Link
- Instant access to full article PDF
Prices may be subject to local taxes which are calculated during checkout
Similar content being viewed by others
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Tong, B., Auer, D., Jaye, M. et al. cDNA clones reveal differences between human glial and endothelial cell platelet-derived growth factor A-chains. Nature 328, 619–621 (1987). https://doi.org/10.1038/328619a0
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1038/328619a0
This article is cited by
-
Signals controlling the expression of PDGF
Molecular Biology Reports (1996)
-
Platelet-derived growth factor B-chain gene expression in mesangial cells: Effect of phorbol ester on gene transcription and mRNA stability
Molecular and Cellular Biochemistry (1994)
-
Effects of experimental ureteral obstruction on platelet-derived growth factor-A and type I procollagen expression in fetal metanephric kidneys
Pediatric Nephrology (1994)
-
Recent developments in the structure, function and regulation of platelet-derived growth factor and its receptors
Cytotechnology (1989)
Comments
By submitting a comment you agree to abide by our Terms and Community Guidelines. If you find something abusive or that does not comply with our terms or guidelines please flag it as inappropriate.