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Differentiation state-dependent surface mobilities of two forms of the neural cell adhesion molecule

Nature volume 324, pages 462–465 (1986)Cite this article

Abstract

The neural cell adhesion molecule (N-CAM) has been implicated in morphogenetic events during formation of the nervous system1,2. Three forms of N-CAM exist, all glycoprotein chains, of relative molecular masses 180,000 (180K), 140K and 120K (N-CAM180, N-CAM140 and N-CAM120) which are differentially expressed on neural cell types and during development3–5. The three chains are thought to carry similar if not identical amino-acid sequences on their extracellular amino-terminal domains, but differ in the length of their carboxy-terminal cytoplasmic region6–8. They occur in highly sialylated embryonic and less sialylated adult forms9,10. N-CAM180 is selectively expressed in more differentiated neural cells and may play a role in the stabilization of cell contacts5. To investigate this, we have studied in the surface membrane of a mouse neuroblastoma cell line N2A the lateral mobility of the two predominant forms of N-CAM, N-CAM180 and N-CAM140, as a function of differentiation. Here we report that as judged by fringe pattern photobleaching, the surface mobility of N-CAM140 is higher than that of N-CAM180, suggesting an association of N-CAM180 with the cytoskeleton or other stabilizing factors. We also show that brain spectrin, a membrane-cytoskeleton linker protein11, binds only to N-CAM180. The immobilization of N-CAM in differentiated N2A cells is achieved by a shift in expression from N-CAM140 to N-CAM180.

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Author notes

  1. Jean Davoust: European Molecular Biology Laboratory, Postfach 10.2209, 6900 Heidelberg, FRG

Authors and Affiliations

  1. Department of Neurobiology, University of Heidelberg, Im Neuenheimer Feld 364, 6900, Heidelberg, FRG

    G. Elisabeth Pollerberg, Melitta Schachner & Jean Davoust

Authors
  1. G. Elisabeth Pollerberg
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  2. Melitta Schachner
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  3. Jean Davoust
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Pollerberg, G., Schachner, M. & Davoust, J. Differentiation state-dependent surface mobilities of two forms of the neural cell adhesion molecule. Nature 324, 462–465 (1986). https://doi.org/10.1038/324462a0

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