Skip to main content

Thank you for visiting nature.com. You are using a browser version with limited support for CSS. To obtain the best experience, we recommend you use a more up to date browser (or turn off compatibility mode in Internet Explorer). In the meantime, to ensure continued support, we are displaying the site without styles and JavaScript.

  • Letter
  • Published:

The v-fms oncogene induces factor independence and tumorigenicity in CSF-1 dependent macrophage cell line

Nature volume 324, pages 377–380 (1986)Cite this article

Abstract

The McDonough strain of feline sarcoma virus (SM-FeSV) transforms fibroblast cell lines in culture and produces fibrosarcomas in domestic cats1,2. SM-FeSV does not induce haematopoietic malignancies in spite of the fact that its viral oncogene, v-fms, codes for a glycoprotein related to the receptor for the mononuclear phagocyte colony stimulating factor, CSF-1 (refs 3, 4). The v-fms-coded polypeptide includes the complete extracellular domain of the c-fms proto-oncogene product5,6 and retains the ability to bind CSF-1 specifically4. The two molecules have very similar sequences except at their extreme carboxyl terminal ends where 40 amino acids of the c-fms-coded glycoprotein are replaced by 11 unrelated residues in the v-fms product5. Autophosphorylation of the c-fms gene product on tyrosine is enhanced by CSF-1 addition3, whereas phosphorylation of the v-fms-coded glycoprotein appears to be constitutive4. We now show that introduction of the v-fms gene into simian virus-40 (SV40)-immortalized, CSF-1 dependent macrophages renders them independent of CSF-1 for growth and tumourigenic in nude mice. These factor-independent cell lines express unaltered levels of the c-fms product which is down-modulated in response to either CSF-1 or the tumour promoter 12-O-tetradecanoyl-phorbol-13-acetate (TPA). The induction of factor independence by a non-autocrine mechanism suggests that the v-fms product is an unregulated kinase that provides growth stimulatory signals in the absence of ligand.

This is a preview of subscription content, access via your institution

Access options

Buy this article

  • Purchase on Springer Link
  • Instant access to full article PDF
Buy now

Prices may be subject to local taxes which are calculated during checkout

Similar content being viewed by others

References

  1. McDonough, S. K., Larsen, S., Brodey, R. S., Stock, N. D. & Hardy, W. D. Jr Cancer Res. 31, 953–956 (1971).

    CAS  PubMed  Google Scholar 

  2. Sarma, P. S., Sharar, A. L. & McDonough, S. Proc. Soc. exp. Biol. Med. 140, 1365–1368 (1972).

    Article  CAS  Google Scholar 

  3. Sherr, C. J. et al. Cell. 41, 665–676 (1985).

    Article  CAS  Google Scholar 

  4. Sacca, R., Stanley, E. R., Sherr, C. J. & Rettenmier, C. W. Proc. natn. Acad. Sci. U.S.A. 83, 3331–3335 (1986).

    Article  ADS  CAS  Google Scholar 

  5. Coussens, L. et al. Nature 320, 277–280 (1986).

    Article  ADS  CAS  Google Scholar 

  6. Wheeler, E. F. et al. J. Virol. 59, 224–233 (1986).

    Article  CAS  Google Scholar 

  7. Schwarzbaum, S., Halpern, R. & Diamond, B. J. Immun. 132, 1158–1162 (1984).

    CAS  PubMed  Google Scholar 

  8. Morgan, C. J. & Stanley, E. R. Biochem. biophys. Res. Commun. 119, 35–41 (1984).

    Article  CAS  Google Scholar 

  9. Donner, L., Fedele, L. A., Garon, C. F., Anderson, S. J. & Sherr, C. J. J. Virol. 41, 489–500 (1982).

    Article  CAS  Google Scholar 

  10. Mann, R., Mulligan, R. C. & Baltimore, D. Cell 33, 153–159 (1983).

    Article  CAS  Google Scholar 

  11. Roussel, M. F., Rettenmier, C. W., Look, A. T. & Sherr, C. J. Molec. cell. Biol. 4, 1999–2009 (1984).

    Article  CAS  Google Scholar 

  12. Nichols, E. J., Manger, R., Hakomori, S., Herscovics, A. & Rohrschneider, L. R. Molec. cell. Biol. 5, 3467–3475 (1985).

    Article  CAS  Google Scholar 

  13. Anderson, S. J., Furth, M., Wolff, L., Ruscetti, S. K. & Sherr, C. J. J. Virol. 44, 696–702 (1982).

    Article  CAS  Google Scholar 

  14. Furman, W. L. et al. Virology 152, 432–445 (1986).

    Article  CAS  Google Scholar 

  15. Anderson, S. J., Gonda, M. A., Rettenmier, C. W. & Sherr, C. J. J. Virol. 51, 730–741 (1984).

    Article  CAS  Google Scholar 

  16. Barbacid, M. & Lauver, A. V. J. Virol. 40, 812–821 (1981).

    Article  CAS  Google Scholar 

  17. Rettenmier, C. W., Chen, J. H., Roussel, M. F. & Sherr, C. J. Science 228, 320–322 (1985).

    Article  ADS  CAS  Google Scholar 

  18. Guilbert, L. J. & Stanley, E. R. J. biol. Chem. 261, 4024–4032 (1986).

    CAS  PubMed  Google Scholar 

  19. Cooper, J. A., Gould, K. L., Cartwright, C. A. & Hunter, T. Science 231, 1431–1434 (1986).

    Article  ADS  CAS  Google Scholar 

  20. Rosenberg, N. & Baltimore, D. J. exp. Med. 143, 1453–1463 (1976).

    Article  CAS  Google Scholar 

  21. Pierce, J. H. & Aaronson, S. A. J. Virol. 46, 993–1002 (1983).

    Article  CAS  Google Scholar 

  22. Adkins, B., Leutz, A. & Graf, T. Cell 39, 439–445 (1984).

    Article  CAS  Google Scholar 

  23. Kahn, P., Adkins, B., Beug, H. & Graf, T. Proc. natn. Acad. Sci. U.S.A. 81, 7122–7126 (1984).

    Article  ADS  CAS  Google Scholar 

  24. Oliff, A., Agranovsky, O., McKinney, M.D., Murty, V. V. V. S. & Bauchwitz, R. Proc. natn. Acad. Sci. U.S.A. 82, 3306–3310 (1985).

    Article  ADS  CAS  Google Scholar 

  25. Cook, W. D., Metcalf, D., Nicola, N. A., Burgess, A. W. & Walker, F. Cell 41, 677–683 (1985).

    Article  CAS  Google Scholar 

  26. Pierce, J. H. et al. Cell 41, 685–693 (1985).

    Article  CAS  Google Scholar 

  27. Andersen, S. M., Klinken, S. P. & Hankins, W. D. Molec. cell. Biol. 5, 3369–3375 (1985).

    Article  Google Scholar 

  28. Downward, J. et al. Nature 307, 521–527 (1984).

    Article  ADS  CAS  Google Scholar 

  29. Ullrich, A. et al. Nature 309, 418–425 (1984).

    Article  ADS  CAS  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Department of Tumor Cell Biology, St Jude Children's Research Hospital, 332 N. Lauderdale, Memphis, Tennessee, 38105, USA

    Esther F. Wheeler, Carl W. Rettenmier, A. Thomas Look & Charles J. Sherr

Authors
  1. Esther F. Wheeler
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Carl W. Rettenmier
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. A. Thomas Look
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Charles J. Sherr
    View author publications

    You can also search for this author in PubMed Google Scholar

Rights and permissions

Reprints and permissions

About this article

Cite this article

Wheeler, E., Rettenmier, C., Look, A. et al. The v-fms oncogene induces factor independence and tumorigenicity in CSF-1 dependent macrophage cell line. Nature 324, 377–380 (1986). https://doi.org/10.1038/324377a0

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1038/324377a0

This article is cited by

Comments

By submitting a comment you agree to abide by our Terms and Community Guidelines. If you find something abusive or that does not comply with our terms or guidelines please flag it as inappropriate.

Search

Advanced search

Quick links

Nature Briefing

Sign up for the Nature Briefing newsletter — what matters in science, free to your inbox daily.

Get the most important science stories of the day, free in your inbox. Sign up for Nature Briefing