Abstract
We have recently derived a series of cloned cell lines1 displaying natural killer (NK) cell-like activity from normal human fetal blood (25 weeks)2. The lines were obtained after repeated stimulation of mononuclear cells with allogeneic Epstein–Barr virus (EBV)-transformed B lymphocytes and are interleukin-2 (IL-2) dependent. Initial characterization of the clones has been reported previously1. Certain of these clones have been found to have unusual surface characteristics, namely, they are recognized by several well-defined anti-T3 antibodies, but do not react with WT31 (ref. 3), which is thought to recognise an invariant epitope of the human (Ti-αβ) structure4,5. Transcription of the genes encoding the α- and β-chains of the T-cell receptor was assessed in two of these clones (F6A4 and F6C7). Ti-β genes were found to be expressed, whereas α messenger RNA was not detected in Northern blot analysis. These data strongly suggest that these cells do not produce a stoichiometric T3/Ti-αβ receptor complex. However, experiments performed with a monoclonal antibody (anti-NKFi) developed against F6C7 cells demonstrated the existence of a unique clonotypic structure [relative molecular mass (Mr) 85,000 (85K)] which is surface-associated with T3 proteins. Furthermore, both anti-T3 and anti-NKFi were found to block cytotoxic effector function. Together, the results support the view that T3 proteins are involved in non-major histocompatibility complex (MHC)-restricted cytotoxic reactions mediated by certain circulating fetal lymphocytes which are likely to use a clonotypic structure distinct from both the ‘first’ (αβ)4 and the putative ‘second’ (γδ)4 T-cell receptor to recognize their target. The present studies were designed to characterize this structure.
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References
Nowill, A. et al. J. exp. Med. 163, 1601–1606 (1986).
Desmonts, G. et al. Lancet i, 500–504 (1985).
Tax, W. et al. Nature 304, 445–447 (1983).
Meuer, S. C. et al. Nature 303, 808–812 (1983).
Spits, H. et al. J. Immun. 135, 1922–1926 (1985).
Brenner, M. B. et al. Nature 322, 145–149 (1986).
Brenner, M. B., Trowbridge, I. S. & Strominger, J. L. Cell 40, 183–190 (1985).
Hercend, T. et al. J. exp. Med. 158, 1547–1553 (1983).
Ritz, J. et al. Science 228, 1540–1544 (1985).
Bank, I. et al. Nature 322, 179–181 (1986).
Hercend, T. et al. J. clin. Invest. 75, 932–943 (1985).
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Moingeon, P., Ythier, A., Goubin, G. et al. A unique T-cell receptor complex expressed on human fetal lymphocytes displaying natural-killer-like activity. Nature 323, 638–640 (1986). https://doi.org/10.1038/323638a0
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DOI: https://doi.org/10.1038/323638a0
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