Abstract
Dissection and reconstitution of the adenovirus DNA replication machinery has led to the discovery of two HeLa nuclear proteins which are required in conjunction with three viral proteins1–4. One of these, nuclear factor I (NF-I), recognizes an internal region of the origin between nucleotides 25 and 40 (refs 4–8) and by binding to one side of the helix stimulates the initiation reaction up to 30-fold. NFI-binding sites have been observed upstream of several cellular genes, such as chicken lysozyme9,10, human IgM11 and human c-myc12, and coincide in most cases with DNase I hypersensitive regions. Here we report the identification of a novel DNA-binding protein from HeLa nuclei, designated NF-III, that recognizes a sequence in the adenovirus origin very close to the NFI-binding site, between nucleotides 36 and 54. This sequence includes the partially conserved nucleotides TATGATAATGAG. NF-III stimulates DNA replication four- to sixfold by increasing the initiation efficiency. Potential cellular binding sites include promoter elements of the histone H2B gene, the human interferon β gene, the human and mouse immunoglobulin Vκ andVHgenes and the mammal/chicken/Xenopus laevis U1 and U2 small nuclear RNA genes. Furthermore, a subset of the herpes simplex virus immediate early promoter specific TAATGARAT elements is homologous with the adenovirus 2 (Ad-2) NFIII-binding site.
This is a preview of subscription content, access via your institution
Access options
Subscribe to this journal
Receive 51 print issues and online access
$199.00 per year
only $3.90 per issue
Buy this article
- Purchase on Springer Link
- Instant access to full article PDF
Prices may be subject to local taxes which are calculated during checkout
Similar content being viewed by others
References
Challberg, M. D. & Kelly, T. J. A. Rev. Biochem. 51, 907–934 (1982).
Sussenbach, J. S. & Van der Vliet, P. C. Curr. Top. Microbiol. Immun. 109, 53–73 (1983).
Nagata, K., Guggenheimer, R. A., Enomoto, T., Lichy, J. H. & Hurwitz, J. Proc. natn. Acad. Sci. U.S.A. 79, 6438–6442 (1982).
Nagata, K., Guggenheimer, R. A. & Hurwitz, J. Proc. natn. Acad. Sci. U.S.A. 80, 4266–4270 (1983).
Rawlins, D. R., Rosenfeld, P. J., Wides, R. J., Challberg, M. D. & Kelly, T. J. Cell 37, 309–319 (1984).
Guggenheimer, R. A., Stillman, B. W., Nagata, K., Tamanoi, F. & Hurwitz, J. Proc. natn. Acad. Sci. U.S.A. 81, 3069–3073 (1984).
Leegwater, P. A. J., Van Driel, W. & Van der Vliet, P. C. EMBO J. 4, 1515–1521 (1985).
De Vries, E., Van Driel, W., Tromp, M., Van Boom, J. H. & Van der Vliet, P. C. Nucleic Acids Res. 13, 4935–4952 (1985).
Borgmeyer, U., Nowock, J. & Sippel, A. E. Nucleic Acids Res. 12, 4295–4311 (1984).
Nowock, J., Borgmeyer, U., Püschel, A. W., Rupp, R. A. W. & Sippel, A. E. Nucleic Acids Res. 13, 2045–2061 (1985).
Hennighausen, L. et al. Nature 314, 289–292 (1985).
Siebenlist, U., Hennighausen, L., Battey, J. & Leder, P. Cell 37, 381–391 (1984).
Sillman, B. W. Cell 35, 7–9 (1983).
Pruijn, G. J. M., Kusters, H. G., Gmelig Meyling, F. H. J. & Van der Vliet, P. C. Eur. J. Biochem. 154, 363–370 (1986).
Tamanoi, F. & Stillman, B. W. Proc. natn. Acad. Sci. U.S.A. 79, 2221–2225 (1982).
Van Bergen, B. G. M., Van der Ley, P. A., Van Driel, W., Van Mansfeld, A. D. M. & Van der Vliet, P. C. Nucleic Acids Res. 11, 1975–1989 (1983).
Leegwater, P. A. J., Van der Vliet, P. C., Rupp, R. A. W., Nowock, J. & Sippel, A. E. EMBO J. 5, 381–386 (1986).
Enomoto, T., Lichy, J. H., Ikeda, J.-E. & Hurwitz, J. Proc. natn. Acad. Sci. U.S.A. 78, 6779–6783 (1981).
Harvey, R. P., Robins, A. J. & Wells, J. R. E. Nucleic Acids Res. 10, 7851–7863 (1982).
Falkner, F. G. & Zachau, H. G. Nature 310, 71–74 (1984).
Fujita, T., Ohno, S., Yasumitsu, H. & Taniguchi, T. Cell 41, 489–496 (1985).
Krol, A., Lund, E. & Dahlberg, J. E. EMBO J. 4, 1529–1535 (1985).
Ciliberto, G., Buckland, R., Cortese, R. & Philipson, L. EMBO J. 4, 1537–1543 (1985).
Whitton, J. L. & Clements, J. B. Nucleic Acids Res. 12, 2061–2079 (1984).
Hay, R. T. EMBO J. 4, 421–426 (1985).
Wang, K. & Pearson, G. D. Nucleic Acids Res. 13, 5173–5187 (1985).
Hay, R. T. J. molec. Biol. 186, 129–136 (1985).
Pearson, G. D. et al. Gene 23, 293–305 (1983).
Rijnders, A. W. M., Van Bergen, B. G. M., Van der Vliet, P. C. & Sussenbach, J. S. Nucleic Acids Res. 11, 8777–8789 (1983).
Tsernoglou, D., Tucker, A. D. & Van der Vliet, P. C. J. molec. Biol. 172, 237–239 (1984).
Van der Vliet, P. C., Van Dam, D. & Kwant, M. M. FEBS Lett. 171, 5–8 (1984).
Challberg, M. D. & Kelly, T. J. Proc. natn. Acad. Sci. U.S.A. 76, 655–659 (1979).
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Pruijn, G., van Driel, W. & van der Vliet, P. Nuclear factor III, a novel sequence-specific DNA-binding protein from HeLa cells stimulating adenovirus DNA replication. Nature 322, 656–659 (1986). https://doi.org/10.1038/322656a0
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1038/322656a0
This article is cited by
-
An insertion and deletion mutant of adenovirus in Muscovy ducks
Archives of Virology (2022)
-
Assembly and remodeling of viral DNA and RNA replicons regulated by cellular molecular chaperones
Biophysical Reviews (2018)
-
Diversity among POU transcription factors in chromatin recognition and cell fate reprogramming
Cellular and Molecular Life Sciences (2018)
-
Early expression of herpes simplex virus (HSV) proteins and reactivation of latent infection
Folia Microbiologica (2000)
Comments
By submitting a comment you agree to abide by our Terms and Community Guidelines. If you find something abusive or that does not comply with our terms or guidelines please flag it as inappropriate.