Abstract
Splicing of the Tetrahymena ribosomal intron was first studied by Cech et al.1,2, who subsequently demonstrated that the intron RNA catalyses its own excision from a primary transcript to yield mature ribosomal RNA3–5. This intron shares several short conserved sequences and a common secondary structure with several other introns 6–9, some of which have also been shown to self-splice10,11. Here I show that the conserved core of the Tetrahymena intron can act in trans to catalyse the sequence-specific cleavage and addition of guanosine to a separate RNA substrate.
This is a preview of subscription content, access via your institution
Access options
Subscribe to this journal
Receive 51 print issues and online access
$199.00 per year
only $3.90 per issue
Buy this article
- Purchase on Springer Link
- Instant access to full article PDF
Prices may be subject to local taxes which are calculated during checkout
Similar content being viewed by others
References
Zaug, A. J. & Cech, T. R. Cell 19, 331–338 (1980).
Grabowski, P. J., Zaug, A. J. & Cech, T. R. Cell 23, 467–476 (1981).
Kruger, K. et al. Cell 31, 147–157 (1982).
Zaug, A. J., Grabowski, P. J. & Cech, T. R. Nature 301, 578–583 (1983).
Zaug, A. J., Kent, J. R. & Cech, T. R. Science 224, 574–578 (1984).
Davies, R. W., Waring, R. B., Ray, J. A., Brown, T. A. & Scazzochio, C. Nature 300, 719–724 (1982).
Michel, F. & Dujon, B. EMBO J. 2, 33–38 (1983).
Cech, T. R. et al. Proc. natn. Acad. Sci. U.S.A. 80, 3903–3907 (1983).
Waring, R. B., Scazzochio, C., Brown, T. A. & Davies, R. W. J. molec. Biol. 167, 595–605 (1983).
Garriga, G. & Lambowitz, A. M. Cell 38, 631–641 (1984).
Horst, G.v.d. & Tabak, H. F. Cell 40, 759–766 (1985).
Cech, T. R., Zaug, A. R. & Grabowski, P. J. Cell 27, 487–496 (1981).
Cech, T. R. Cell 34, 713–716 (1983).
Bass, B. & Cech, T. R. Nature 308, 820–826 (1984).
Inoue, T. & Cech, T. R. Proc. natn. Acad. Sci. U.S.A. 82, 648–652 (1985).
Tanner, N. K. & Cech, T. R. Nucleic Acids Res. 13, 7759–7779 (1985).
Sharp, P. A. Cell 42, 397–400 (1985).
Zaug, A.J. & Cech, T. R. Science 231, 470–475 (1986).
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Szostak, J. Enzymatic activity of the conserved core of a group I self-splicing intron. Nature 322, 83–86 (1986). https://doi.org/10.1038/322083a0
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1038/322083a0
This article is cited by
-
Cryo-EM reveals dynamics of Tetrahymena group I intron self-splicing
Nature Catalysis (2023)
-
In Vitro Selection of Short DNA Aptamers that Can Inhibit or Alleviate Cocaine and MK-801 Inhibition of Muscle-Type Nicotinic Acetylcholine Receptors
The Journal of Membrane Biology (2022)
-
Minimal RNA Aptamer Sequences That Can Inhibit or Alleviate Noncompetitive Inhibition of the Muscle-Type Nicotinic Acetylcholine Receptor
Journal of Membrane Biology (2010)
-
Approaches for the sequence-specific knockdown of mRNA
Nature Biotechnology (2003)
-
A novel regulator inhibits HBV gene expression
Journal of Biomedical Science (1998)
Comments
By submitting a comment you agree to abide by our Terms and Community Guidelines. If you find something abusive or that does not comply with our terms or guidelines please flag it as inappropriate.