Abstract
Agrobacterium rhizogenes provokes neoplastic outgrowths on higher plants by transferring two small regions from the root-inducing (Ri) plasmid to the host genome1–3. Homology with the left transferred DNA (TL DNA) was detected in uninfected Nicotiana glauca1 and a molecular clone (λNg31) of this region was obtained from N. glauca4. We report here that the genome of N. glauca contains a large inverted repeat which is homologous with the single copy TL DNA of A. rhizogenes. Related sequences are present in several Nicotiana species, but are absent in others. The DNA sequence of the centre of the inverted repeat has been compared with the corresponding region from the bacterium. Both contain open reading frames which could potentially produce similar polypeptides. The data strongly suggest that an Agrobacterium infection resulted in genetic transformation early in the evolution of the genus Nicotiana.
This is a preview of subscription content, access via your institution
Access options
Subscribe to this journal
Receive 51 print issues and online access
$199.00 per year
only $3.90 per issue
Buy this article
- Purchase on SpringerLink
- Instant access to full article PDF
Prices may be subject to local taxes which are calculated during checkout
Similar content being viewed by others
References
White, F. F., Ghidossi, G., Gordon, M. P. & Nester, E. W. Proc. natn. Acad. Sci. U.S.A. 79, 3193–3197 (1982).
Huffman, G. A., White, F. F., Gordon, M. P. & Nester, E. W. J. Bact. 157, 269–276 (1984).
White, F. F., Taylor, B. H., Huffman, G. A., Gordon, M. P. & Nester, E. W. J. Bact. 164, 33–44 (1985).
White, F. F., Garfinkel, D. J., Huffman, G. A., Gordon, M. P. & Nester, E. W. Nature 310, 348–350 (1983).
Taylor, B. H., White, F. F., Nester, E. W. & Gordon, M. P. Molec. gen. Genet. (in the press).
Kwok, W. W., Nester, E. W. & Gordon, M. P. Nucleic Acids Res. 13, 459–471 (1985).
Costantino, P., Spano, Pomponi, M., Benvenuto, E. & Ancora, G. J. Molec. appl. Genet. 2, 465–470 (1984).
Tepfer, D. Cell 37, 959–967 (1984).
Barker, R. F., Idler, K. B., Thompson, D. V. & Kemp, J. D. Pl. molec. Biol. 2, 335–350 (1983).
Lahners, K., Byrne, M. C. & Chilton, M.-D. Plasmid 11, 130–140 (1984).
Spano, L., Pomponi, M., Costantino, P., Van Slogteren, G. M. S. & Tempe, J. Pl. molec. Biol. 1, 291–300 (1982).
Thomashow, M. F., Nutter, R., Montoya, A. L., Gordon, M. P. & Nester, E. W. Cell 19, 729–739 (1980).
Vieira, J. & Messing, J. Gene 19, 259–268 (1982).
Sanger, F., Nicklen, S. & Coulson, A. R. Proc. natn. Acad. Sci. U.S.A. 74, 5463–5467 (1977).
Guo, L.-H. & Wu, R. Nucleic Acids Res. 10, 2065–2084 (1982).
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Furner, I., Huffman, G., Amasino, R. et al. An Agrobacterium transformation in the evolution of the genus Nicotiana. Nature 319, 422–427 (1986). https://doi.org/10.1038/319422a0
Received:
Accepted:
Published:
Issue Date:
DOI: https://doi.org/10.1038/319422a0
This article is cited by
-
Upscaling biomass production of rosmarinic acid-rich hairy root cultures of Agastache rugosa (Fisch. & C.A.Mey.) Kuntze
Plant Cell, Tissue and Organ Culture (PCTOC) (2024)
-
Extensive natural Agrobacterium-induced transformation in the genus Camellia
Planta (2023)
-
Ancient horizontal gene transfer from Rhizobium rhizogenes to European genera of the Figwort family (Scrophulariaceae)
Euphytica (2020)
-
Plant tumors: a hundred years of study
Planta (2020)
-
Effects of methyl jasmonate on the growth and triterpenoid production of diploid and tetraploid Centella asiatica (L.) Urb. hairy root cultures
Scientific Reports (2019)
Comments
By submitting a comment you agree to abide by our Terms and Community Guidelines. If you find something abusive or that does not comply with our terms or guidelines please flag it as inappropriate.