Skip to main content

Thank you for visiting nature.com. You are using a browser version with limited support for CSS. To obtain the best experience, we recommend you use a more up to date browser (or turn off compatibility mode in Internet Explorer). In the meantime, to ensure continued support, we are displaying the site without styles and JavaScript.

  • Letter
  • Published:

Chromosomal localization of a unique gene by non-autoradiographic in situ hybridization

Nature volume 317, pages 175–177 (1985)Cite this article

Abstract

During the past few years, several methods have been developed for the detection of specific nucleic acid sequences by in situ hybridization using non-radioactive labels such as fluorochromes, cytochemically detectable enzymes and electron-dense markers1–4. These methods are preferable to autoradiography in terms of speed of performance and topological resolution. Their limited sensitivity, however, has so far restricted their use to the detection of repeated sequences. Here we report single gene detection with a procedure using 2-acetylaminofluorene (AFF)-modified probes, immunoperoxidase cytochemistry and reflection-contrast microscopy. We confirmed the autoradiographic data on the localization of the human thyroglobulin (Tg) gene to the distal end of the long arm of chromosome 8 (ref. 5). A mixture of cosmid cHT2-derived subclones of the 3′ part of the Tg gene, 22.3 kilobase pairs (kbp) in total, was used as a hybridization probe. This procedure can be used to map other unique sequences, if genomic clones are available from which clones with an appropriate amount of inserts can be isolated.

This is a preview of subscription content, access via your institution

Access options

Buy this article

  • Purchase on Springer Link
  • Instant access to full article PDF
Buy now

Prices may be subject to local taxes which are calculated during checkout

Similar content being viewed by others

References

  1. Rudkin, G. T. & Stollar, B. D. Nature 265, 472–473 (1977).

    Article  ADS  CAS  Google Scholar 

  2. Langer-Safer, P. R., Levine, M. & Ward, D. C. Proc. natn. Acad. Sci. U.S.A. 79, 4381–4385 (1982).

    Article  ADS  CAS  Google Scholar 

  3. Bauman, J. G. J. Van der Ploeg, M. & Van Duijn, P. in Investigative Microtechniques in Medicine and Biology (eds Chayen, J. & Bitensky, L.) 41–87 (Dekker, New York, 1984).

    Google Scholar 

  4. Manuelidis, L., Langer-Safer, P. R. & Ward, D. C. J. Cell Biol. 95, 619–625 (1982).

    Article  CAS  Google Scholar 

  5. Baas, F. et al. Hum. Genet. 69, 138–143 (1985).

    Article  CAS  Google Scholar 

  6. Bauman, J. G. J., Wiegant, J., Borst, P. & Van Duijn, P. Expl Cell Res. 138, 485–490 (1980).

    Article  Google Scholar 

  7. Renz, M. EMBO J. 2, 817–822 (1983).

    Article  CAS  Google Scholar 

  8. Shroyer, K. R. & Nakane, P. K. J. Cell Biol 97, 377a (1983).

    Google Scholar 

  9. Kriek, E., Miller, J. A., Juhl, U. & Miller, E. C. Biochemistry 6, 177–182 (1967).

    Article  CAS  Google Scholar 

  10. Landegent, J. E. Jansen in de Wal, N., Baan, R. A., Hoeijmakers, J. H. J. & Van der Ploeg, M. Expl Cell Res. 153, 61–72 (1984).

    Article  CAS  Google Scholar 

  11. Van Ommen, G.-J. B. et al. Nucleic Acids Res. 11, 2273–2285 (1983).

    Article  ADS  CAS  Google Scholar 

  12. Baas, F., Bikker, H., Van Ommen, G.-J. B. & De Vijlder, J. J. M. Hum. Genet. 67, 301–305 (1984).

    Article  CAS  Google Scholar 

  13. Ploem, J. S. in Mononuclear Phagocytes in Immunity, Infection and Pathology (ed. Van Furth, R.) 405–421 (Blackwell, Oxford, 1975).

    Google Scholar 

  14. Landegent, J. E. Jansen in de Wal, N., Ploem, J. S. & Van der Ploeg, M. J. Histochem. Cytochem. (in the press).

  15. Van der Ploeg, M. & Van Duijn, P. Histochemistry 62, 227–232 (1979).

    Article  CAS  Google Scholar 

  16. Dutrillaux, B. & Lejeune, J. C.r. hebd. Séanc. Acad. Sci., Paris 272, 2638–2640 (1971).

    CAS  Google Scholar 

  17. Sumner, A. T., Evans, H. J. & Buckland, R. A. Nature new Biol. 232, 31–32 (1971).

    Article  CAS  Google Scholar 

  18. Gerhard, D.S., Kawasaki, E.S., Bancroft, F.C. & Szabo, P. Proc. natn. Acad. Sci. U.S.A. 78, 3755–3759 (1981).

    Article  ADS  CAS  Google Scholar 

  19. Harper, M. E., Ullrich, A. & Saunders, G. F. Proc. natn. Acad. Sci. U.S.A. 78, 4458–4460 (1981).

    Article  ADS  CAS  Google Scholar 

  20. Van Prooijen-Knegt, A. C. et al. Expl Cell Res. 141, 397–407 (1982).

    Article  CAS  Google Scholar 

  21. Melton, D. A. et al. Nucleic Acids Res. 12, 7035–7056 (1984).

    Article  CAS  Google Scholar 

  22. Cox, K. H., Deleon, D. V., Angerer, L. M. & Angerer, R. C. Devl Biol. 101, 485–502 (1984).

    Article  CAS  Google Scholar 

  23. Heyting, C., Kroes, W. G. M., Kriek, E., Meyer, I. & Slater, R. Acta histochem. (in the press).

  24. Morton, C. C. et al. Proc. natn. Acad. Sci. U.S.A. 81, 2816–2820 (1984).

    Article  ADS  CAS  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Department of Histochemistry and Cytochemistry, Sylvius Laboratories, State University, 2333 AL, Leiden, The Netherlands

    J. E. Landegent, N. Jansen in de Wal, P. van Duijn & M. van der Ploeg

  2. Department of Anthropogenetics, Sylvius Laboratories, State University, 2333 AL, Leiden, The Netherlands

    G.-J. B. van Omment

  3. Department of Pediatric Endocrinology, Academic Medical Center, 1105 AZ, Amsterdam, The Netherlands

    F. Baas & J. J. M. de Vijlderi

Authors
  1. J. E. Landegent
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. N. Jansen in de Wal
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. G.-J. B. van Omment
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. F. Baas
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. J. J. M. de Vijlderi
    View author publications

    You can also search for this author in PubMed Google Scholar

  6. P. van Duijn
    View author publications

    You can also search for this author in PubMed Google Scholar

  7. M. van der Ploeg
    View author publications

    You can also search for this author in PubMed Google Scholar

Rights and permissions

Reprints and permissions

About this article

Cite this article

Landegent, J., Jansen in de Wal, N., van Omment, GJ. et al. Chromosomal localization of a unique gene by non-autoradiographic in situ hybridization. Nature 317, 175–177 (1985). https://doi.org/10.1038/317175a0

Download citation

  • Received:

  • Accepted:

  • Issue Date:

  • DOI: https://doi.org/10.1038/317175a0

This article is cited by

Comments

By submitting a comment you agree to abide by our Terms and Community Guidelines. If you find something abusive or that does not comply with our terms or guidelines please flag it as inappropriate.

Search

Advanced search

Quick links

Nature Briefing

Sign up for the Nature Briefing newsletter — what matters in science, free to your inbox daily.

Get the most important science stories of the day, free in your inbox. Sign up for Nature Briefing