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Amino-acid sequence of a Ca2+ + Mg2+ -dependent ATPase from rabbit muscle sarcoplasmic reticulum, deduced from its complementary DNA sequence

Nature volume 316, pages 696–700 (1985)Cite this article

A Corrigendum to this article was published on 01 October 1985

Abstract

We have cloned and sequenced complementary DNA encoding a Ca2+-ATPase of rabbit muscle sarcoplasmic reticulum. We propose a model of the protein which has 3 cytoplasmic domains joined to a set of 10 transmembrane helices by a narrow, penta-helical stalk. In this model. ATP bound to one cytoplasmic domain would phosphorylate an aspartate in an adjoining cytoplasmic domain, inducing translocation of Ca2+ from binding sites on the stalk.

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Authors and Affiliations

  1. Banting and Best Department of Medical Research, Charles H. Best Institute, University of Toronto, 112 College St, Toronto, Ontario, Canada, M5G 1L6

    David H. MacLennan, Christopher J. Brandl & Bozena Korczak

  2. National Institute for Medical Research, The Ridgeway, Mill Hill, London, NW7 1AA, UK

    N. Michael Green

Authors
  1. David H. MacLennan
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  2. Christopher J. Brandl
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  3. Bozena Korczak
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  4. N. Michael Green
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MacLennan, D., Brandl, C., Korczak, B. et al. Amino-acid sequence of a Ca2+ + Mg2+ -dependent ATPase from rabbit muscle sarcoplasmic reticulum, deduced from its complementary DNA sequence. Nature 316, 696–700 (1985). https://doi.org/10.1038/316696a0

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