Abstract
Acquired immune deficiency syndrome (AIDS) is an epidemic immunosuppressive disease characteristically associated with a depletion of T lymphocytes of the helper/inducer phenotype1. Numerous converging lines of research have implicated a human T-cell lymphotropic retrovirus, HTLV-III, in the pathogenesis of AIDS2–5. Recently, several distinct forms of the HTLV-III genome were molecularly cloned in phage and extensively characterized6,7. In the present study, a clone containing full-length HTLV-III proviral DNA7 was inserted into a plasmid and used to transfect cord blood T cells from normal newborn humans. We demonstrate that this molecular clone is infectious in vitro and causes marked cytopathic effects on T-cell cultures. This is the first direct evidence that the HTLV-III genome, rather than a minor component of the virus complex, is cytopathic for T cells. Using this biologically competent clone and mutants derived from it, it should now be possible to localize the subgenomic regions that contribute to the biological effects of HTLV-III.
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Fisher, A., Collalti, E., Ratner, L. et al. A molecular clone of HTLV-III with biological activity. Nature 316, 262–265 (1985). https://doi.org/10.1038/316262a0
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DOI: https://doi.org/10.1038/316262a0
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