Abstract
The study of retrovirus-induced leukaemias in mice is a powerful tool for the elucidation of the normal regulation of the haematopoietic system. The acute murine spleen focus-forming viruses (SFFV) can be classified according to the haematopoietic lineage on which they exert their effects in the adult mouse. Here we report a new SFFV isolate, the AF-1 virus, with the novel ability to transform cells of the mononuclear phagocyte lineage. The virus was isolated from sarcomas that were induced on passage of a cloned Friend helper virus (F-MuLV, 643/22F)1 in newborn BALB/c mice. We have cloned the transforming defective subunit of the AF-1 viral complex in NRK cells and isolated several subclones. Analysis of the proviral genome in two non-producer cell clones reveals that AF-1 virus contains Harvey v-ras-specific sequences (Fig. 1). Thus, AF-1 virus is closely related to Harvey murine sarcoma virus (Ha-MSV)2, and is, at present, the only tool by which permanent cell lines can be obtained from mononuclear phagocytes in the mouse.
This is a preview of subscription content, access via your institution
Access options
Subscribe to this journal
Receive 51 print issues and online access
$199.00 per year
only $3.90 per issue
Buy this article
- Purchase on Springer Link
- Instant access to full article PDF
Prices may be subject to local taxes which are calculated during checkout
Similar content being viewed by others
References
Ostertag, W. et al. J. Virol. 33, 573–582 (1980).
Harvey, J. J. Nature 204, 1104–1105 (1964).
Huhn, D. & Meister, P. Cancer 42, 1341–1349 (1978).
Rappaport, H. Tumors of the Hemopoietic System. Atlas of Tumor Pathology 49–63 (Armed Forces Institute of Pathology, Washington, DC, 1966).
Vardiman, J. W., Byrne, G. E. & Rappaport, H. Cancer 36, 419–427 (1975).
Scott, R. B. & Robb-Smith, A. H. T. Lancet ii, 194–198 (1939).
Johnson, G. R., Ostertag, W. & Nicola, N. A. in Modern Trends in Human Leukemia (ed. Neth, R.) (in the press).
Ostertag, W. & Pragnell, I. B. Curr. Topics Microbiol. Immun. 94/95, 143–208 (1981).
Dannenberg, A. M. Jr & Suga, M. in Methods for Studying Mononuclear Phagocytes (eds Adams, D. O., Edelson, P. J. & Koren, H. S.) 375–396 (Academic, New York, 1981).
Leder, L. D. Der Blutmonozyt (Springer, Berlin, 1967).
Springer, T., Galfre, G., Secker, D. S. & Milstein, C. Eur. J. Immun. 8, 539–551 (1978).
Flotte, T. J., Springer, T. A. & Thorbecke, J. Am. J. Path. 111, 112–124 (1983).
Taffet, S. M. & Russell, S. W. in Methods for Studying Mononuclear Phagocytes (eds Adams, D. O., Edelson, P. J. & Koren, H. S.) 283 (Academic, New York, 1981).
Lancet i, 455–456 (1983).
Iscove, N. N., Guilbert, L. J. & Weyman, C. Expl Cell Res. 126, 121–126 (1980).
Iscove, N. N., Roitsch, C. A., Williams, N. & Guilbert, L. J. J. cell. Physiol. (Suppl.) l, 65–71 (1982).
Southern, E. M. J. molec. Biol. 98, 503–517 (1975).
Wahl, G. M. et al. Proc. natn. Acad. Sci. U.S.A. 76, 3683–3687 (1979).
Ellis, R. W. et al. Nature 292, 506–510 (1981).
Bassin, R. H., Simons, P. J., Chesterman, F. C. & Harvey, J. J. Int. J. Cancer 3, 265–272 (1968).
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Franz, T., Löhler, J., Fusco, A. et al. Transformation of mononuclear phagocytes in vivo and malignant histiocytosis caused by a novel murine spleen focus-forming virus. Nature 315, 149–151 (1985). https://doi.org/10.1038/315149a0
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1038/315149a0
Comments
By submitting a comment you agree to abide by our Terms and Community Guidelines. If you find something abusive or that does not comply with our terms or guidelines please flag it as inappropriate.
