Skip to main content

Thank you for visiting nature.com. You are using a browser version with limited support for CSS. To obtain the best experience, we recommend you use a more up to date browser (or turn off compatibility mode in Internet Explorer). In the meantime, to ensure continued support, we are displaying the site without styles and JavaScript.

  • Article
  • Published:

Identification and comparison of two sequence elements that confer cell-type specific transcription in yeast

Nature volume 314, pages 598–603 (1985)Cite this article

An Erratum to this article was published on 06 June 1985

Abstract

The MATα2 protein of budding yeast represses a set of genes; if the MATa1 protein is also present, a further set of genes is repressed. DNA sequence comparisons reveal a 20-base pair ‘operator’ sequence that is present in genes repressed by a1/α2. A related, but distinct, sequence is found in genes repressed by α2 alone.

This is a preview of subscription content, access via your institution

Access options

Buy this article

  • Purchase on Springer Link
  • Instant access to full article PDF
Buy now

Prices may be subject to local taxes which are calculated during checkout

Similar content being viewed by others

References

  1. Mortimer, R. K. & Hawthorne, D. C. Yeasts 1, 385 (1969).

    Google Scholar 

  2. Mackay, V. L. & Manney, T. R. Genetics 76, 255–271 (1974).

    CAS  PubMed  PubMed Central  Google Scholar 

  3. Mackay, V. L. & Manney, T. R. Genetics 76, 273–288 (1974).

    CAS  PubMed  PubMed Central  Google Scholar 

  4. Nasmyth, K. A., Tatchell, K., Hall, B., Astell, C. R. & Smith, M. Nature 289, 244–250 (1981).

    Article  ADS  CAS  Google Scholar 

  5. Tatchell, K. et al. Cell 27, 25–35 (1981).

    Article  CAS  Google Scholar 

  6. Strathern, J. N., Hicks, J. B. & Herskowitz, I. J. molec. Biol. 147, 357–372 (1981).

    Article  CAS  Google Scholar 

  7. Klar, A. J. S., Strathern, J. N. & Hicks, J. B. in Microbial Development (eds Losick, R. & Shapiro L.) 151–195 (Cold Spring Harbor Laboratory, New York, 1984).

    Google Scholar 

  8. Wilson, K. & Herskowitz, I. Molec. cell. Biol. 4, 2420–2427 (1984).

    Article  CAS  Google Scholar 

  9. Klar, A. J. S., Strathern, J. N., Broach, J. B. & Hicks, J. B. Nature 289, 239–244 (1981).

    Article  ADS  CAS  Google Scholar 

  10. Jensen, R., Sprague, G. F. & Herskowitz, I. Proc. natn. Acad. Sci. U.S.A. 80, 3055–3039 (1983).

    ADS  Google Scholar 

  11. Shepherd, J. C. W., McGinnis, W., Carrasco, A. E., de Robertis, E. M. & Gehring, W. J. Nature 310, 70–71 (1984).

    Article  ADS  CAS  Google Scholar 

  12. McGinnis, W., Levine, M. S., Hafen, E., Kuroiwa, A. & Gehring, W. J. Nature 308, 428–433 (1984).

    Article  ADS  CAS  Google Scholar 

  13. Scott, M. P. & Weiner, A. J. Proc. natn. Acad. Sci. U.S.A. 81, 4115–4119 (1984).

    Article  ADS  CAS  Google Scholar 

  14. Poole, S. J., Kauvar, L. M., Drees, B. & Kornberg, T. Cell 40, 37–43 (1985).

    Article  CAS  Google Scholar 

  15. Carrasco, A. E., McGinnis, W., Gehring, W. J. & de Robertis, E. M. Cell 37, 409–414 (1984).

    Article  CAS  Google Scholar 

  16. McGinnis, W., Garber, R. L., Wirz, J., Kuroiwa, A. & Gehring, W. J. Cell 37, 403–408 (1984).

    Article  CAS  Google Scholar 

  17. Sauer, R. T., Yocum, R. R., Doolittle, R. F., Lewis, M. & Pabo, C. O. Nature 298, 447–451 (1982).

    Article  ADS  CAS  Google Scholar 

  18. Kostriken, R., Strathern, J. N., Klar, A. J. S., Hicks, J. B. & Heffron, F. Cell 35, 167–174 (1983).

    Article  CAS  Google Scholar 

  19. Jensen, R. thesis, Univ. Oregon (1983).

  20. Nasmyth, K. A. Cell (submitted).

  21. Miller, A. M., Sternglanz, R. & Nasmyth, K. A. Cold Spring Harb. Symp. quant. Biol. 49, 105–113 (1984).

    Article  CAS  Google Scholar 

  22. Siliciano, P. & Tatchell, K. Cell 37, 969–978 (1984).

    Article  CAS  Google Scholar 

  23. Nasmyth, K. A. Cell (submitted).

  24. Guarente, L. & Ptashne, M. Proc. natn. Acad. Sci. U.S.A. 78, 2199–2203 (1981).

    Article  ADS  CAS  Google Scholar 

  25. Guarente, L., Lalonde, B., Gifford, P. & Alani, E. Cell 36, 503–511 (1984).

    Article  CAS  Google Scholar 

  26. Staden, R. Nucleic Acids Res. 12, 505–519 (1984).

    Article  CAS  Google Scholar 

  27. Singh, A. et al. Nucleic Acids Res 11, 4049–4063 (1983).

    Article  CAS  Google Scholar 

  28. Guarente, L. Cell 799–800 (1984).

    Article  CAS  Google Scholar 

  29. Pribnow, D. Biol. regul. Dev. 1, 219–277 (1979).

    Article  CAS  Google Scholar 

  30. Brent, R. & Ptashne, M. Nature 312, 612–615 (1984).

    Article  ADS  CAS  Google Scholar 

  31. Laughon, A & Scott, M. P. Nature 310, 25–30 (1984).

    Article  ADS  CAS  Google Scholar 

  32. Struhl, G. Proc. natn. Acad. Sci. U.S.A. 79, 7380–7384 (1982).

    Article  ADS  CAS  Google Scholar 

  33. Lawrence, P. A. & Morata, G. Cell 35, 595–601 (1983).

    Article  CAS  Google Scholar 

  34. Astell, C. R. et al. Cell 27, 15–23 (1981).

    Article  CAS  Google Scholar 

  35. Staden, R. Nucleic Acids Res. 10, 2951–2961 (1982).

    Article  CAS  Google Scholar 

  36. Guarente, L. & Mason, T. Cell 32, 1279–1286 (1983).

    Article  CAS  Google Scholar 

  37. Messing, J. & Vieira, J. Gene 19, 269–276 (1982).

    Article  CAS  Google Scholar 

  38. Maniatis, T., Fritsch, E. F. & Sambrook, J. in Molecular Cloning: A Laboratory Manual, 315 (Cold Spring Harbor Laboratory, New York, 1982).

    Google Scholar 

  39. Miller, J. H. in Experiments in Molecular Genetics, 352–355 (Cold Spring Harbor Laboratory, New York, 1972).

    Google Scholar 

  40. Nasmyth, K. A. Nature 302, 670–676 (1983).

    Article  ADS  CAS  Google Scholar 

  41. Rothstein, R. Meth. Enzym. 101, 202 (1983).

    Article  CAS  Google Scholar 

  42. Bankier, A. T. & Barrell, B. G. in Techniques in Life Sciences Vol. B508, 1–34 (Elsevier, Amsterdam, 1983).

    Google Scholar 

Download references

Author information

Author notes

  1. Vivian L. MacKay: Zymogenetics Corporation, 2121 North 35th Street, Seattle, Washington 98103, USA

Authors and Affiliations

  1. MRC Laboratory of Molecular Biology, Hills Road, Cambridge, CB2 2QH, UK

    Allan M. Miller, Vivian L. MacKay & Kim A. Nasmyth

Authors
  1. Allan M. Miller
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Vivian L. MacKay
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Kim A. Nasmyth
    View author publications

    You can also search for this author in PubMed Google Scholar

Rights and permissions

Reprints and permissions

About this article

Cite this article

Miller, A., MacKay, V. & Nasmyth, K. Identification and comparison of two sequence elements that confer cell-type specific transcription in yeast. Nature 314, 598–603 (1985). https://doi.org/10.1038/314598a0

Download citation

  • Received:

  • Accepted:

  • Issue Date:

  • DOI: https://doi.org/10.1038/314598a0

This article is cited by

Comments

By submitting a comment you agree to abide by our Terms and Community Guidelines. If you find something abusive or that does not comply with our terms or guidelines please flag it as inappropriate.

Search

Advanced search

Quick links

Nature Briefing

Sign up for the Nature Briefing newsletter — what matters in science, free to your inbox daily.

Get the most important science stories of the day, free in your inbox. Sign up for Nature Briefing