Abstract
Class I genes of the major histocompatibility complex (MHC) appear to be activated in mouse cells transformed by the DNA tumour virus simian virus 40 (SV40)1. Conversely, suppression of MHC class I genes has been reported in adenovirus-12-transformed baby kidney rat cells2. We have now investigated the expression of genes encoded by the rat MHC locus in rat fibroblast cells transformed by polyoma virus (Py). Using a mouse genomic H–2 clone as a probe in Northern transfer hybridization analysis, we have observed a high level of expression of rat MHC class I messenger RNA in all the transformed rat cell lines analysed. The class I 1.6-kilobase (kb) mRNA activated in Py-transformed rat cells appears to contain an Alu-like type II repeat element, as the same 1.6-kb mRNA is detected using either the H–2 class I sequence or a repetitive Alu-like type II element as a probe. High levels of heterogeneous poly(A)+ transcripts of 0.5–0.8 kb are also observed in Py-transformed rat cells using probes containing an Alu-like type II repetitive element.
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Majello, B., Mantia, G., Simeone, A. et al. Activation of major histocompatibility complex class I mRNA containing an Alu-like repeat in polyoma virus-transformed rat cells. Nature 314, 457–459 (1985). https://doi.org/10.1038/314457a0
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DOI: https://doi.org/10.1038/314457a0
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