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High-affinity binding site for a specific nuclear protein in the human IgM gene

Nature volume 314, pages 289–292 (1985)Cite this article

A Corrigendum to this article was published on 01 May 1985

Abstract

Proteins binding to specific regions of DNA with high affinity frequently govern or regulate reactions at the gene level1–8. We have identified a high-affinity binding site in the immunoglobulin μ gene that binds a specific nuclear protein, and have now characterized it fully using nuclear factor 1 (NF-1), a protein purified from the nuclei of HeLa cells9,10 and required for the in vitro replication of adenovirus (Ad) DNA9–12. NF-1 protects a 25-base pair (bp) double-stranded segment of DNA which shares a consensus sequence, 5′ TGGA/CNNNNNGCCAA 3′, with similar binding sites in the Ad-5 terminal repeat10–12 and the human c-myc gene13. Although this site differs from the enhancer region13–16, a biological function is suggested by the fact that it is DNase I hypersensitive in immunoglobulin-producing lymphoblastoid cells. The binding site for the NF-1 protein in the μ gene, by analogy with the site in the Ad-5 terminal repeat, may represent one component of a cellular origin of replication; alternatively, it may be responsible for the activation of the chromatin in this region.

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References

  1. Gilbert, W., Maizels, N. & Maxam, A. Cold Spring Harb. Symp. quant. Biol. 38, 845–855 (1974).

    Article  CAS  Google Scholar 

  2. Maniatis, T. et al. Cell 5, 109–113 (1975).

    Article  CAS  Google Scholar 

  3. Dynan, W. S. & Tjian, R. Cell 35, 79–87 (1983).

    Article  CAS  Google Scholar 

  4. Jones, K. A. & Tjian, R. Cell 36, 155–162 (1984).

    Article  CAS  Google Scholar 

  5. Compton, J. G., Schrader, W. T. & O'Malley, B. W. Proc. nat. Acad. Sci. U.S.A. 80, 16–20 (1983).

    Article  ADS  CAS  Google Scholar 

  6. Karin, M. et al. Nature 308, 513–519 (1984).

    Article  ADS  CAS  Google Scholar 

  7. Renkawitz, R., Schutz, G. von der Ahe, D. & Beato, M. Cell 37, 503–510 (1984).

    Article  CAS  Google Scholar 

  8. Chandler, V. L., Maler, B. A. & Yamamoto, K. R. Cell 33, 489–499 (1983).

    Article  CAS  Google Scholar 

  9. Nagata, K., Guggenheimer, R. A., Enomoto, T., Lichy, J. H. & Hurwitz, J. Proc. natn. Acad. Sci. U.S.A. 79, 6438–6442 (1982).

    Article  ADS  CAS  Google Scholar 

  10. Rawlins, D. R., Rosenfeld, P. J., Wides, R. J., Challberg, M. D. & Kelly, T. J. Jr Cell 37, 309–319 (1984).

    Article  CAS  Google Scholar 

  11. Nagata, K., Guggenheimer, R. A. & Hurwitz, J. Proc. natn. Acad. Sci. U.S.A. 80, 6177–6181 (1983).

    Article  ADS  CAS  Google Scholar 

  12. Guggenheimer, R. A., Stillman, B. W., Nagata, K., Tamanoi, F. & Hurwitz, J. Proc. natn. Acad. Sci. U.S.A. 81, 3069–3073 (1984).

    Article  ADS  CAS  Google Scholar 

  13. Siebenlist, U., Hennighausen, L., Battey, J. & Leder, P. Cell 37, 381–391 (1984).

    Article  CAS  Google Scholar 

  14. Hayday, A. et al. Nature 307, 334–340 (1984).

    Article  ADS  CAS  Google Scholar 

  15. Rabbitts, T. H., Hamlyn, P. H. & Baer, R. Nature 306, 760–765 (1983).

    Article  ADS  CAS  Google Scholar 

  16. Bannerji, J., Olson, L. & Schaffner, W. Cell 33, 729–740 (1983).

    Article  Google Scholar 

  17. Taub, R. et al. Proc. natn. Acad. Sci. U.S.A. 79, 7837–7841 (1982).

    Article  ADS  CAS  Google Scholar 

  18. Leder, P. et al. Science 222, 765–771 (1983).

    Article  ADS  CAS  Google Scholar 

  19. Battey, J. et al. Cell 34, 779–789 (1983).

    Article  CAS  Google Scholar 

  20. Taub, R. et al. Cell 36, 339–348 (1984).

    Article  CAS  Google Scholar 

  21. Riggs, A. D., Bourgeois, S. & Cohn, M. J. molec. Biol. 53, 401–417 (1970).

    Article  CAS  Google Scholar 

  22. Jack, R. S., Gehring, W. J. & Brack, C. Cell 24, 321–333 (1981).

    Article  CAS  Google Scholar 

  23. Borgmeyer, U., Nowock, J. & Sippel, A. E. Nucleic Acids Res. 12, 4295–4311 (1984).

    Article  CAS  Google Scholar 

  24. Pabo, C. & Lewis, A. Nature 298, 443–447 (1982).

    Article  ADS  CAS  Google Scholar 

  25. Shermoen, A. W. & Beckendorf, S. K. Cell 29, 601–607 (1982).

    Article  CAS  Google Scholar 

  26. Emerson, B. M. & Felsenfeld, G. Proc. natn. Acad. Sci. U.S.A. 81, 95–99 (1984).

    Article  ADS  CAS  Google Scholar 

  27. Gronostajski, R. M., Nagata, K. & Hurwitz, J. Proc. natn. Acad. Sci. U.S.A. 81, 4013–4017 (1984).

    Article  ADS  CAS  Google Scholar 

  28. Lewin, B. M. Gene Expression 2 2nd edn (Wiley, New York, 1980).

    Google Scholar 

  29. Leder, P. Sci. Am. 246(5), 102–115 (1982).

    Article  ADS  CAS  Google Scholar 

  30. Shimizu, A. & Honjo, T. Cell 36, 801–803 (1984).

    Article  CAS  Google Scholar 

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Authors and Affiliations

  1. Department of Genetics, Harvard Medical School, Boston, Massachusetts, 02115, USA

    Lothar Hennighausen, Ulrich Siebenlist, David Danner & Philip Leder

  2. Department of Molecular Biology and Genetics, The Johns Hopkins School of Medicine, Baltimore, Maryland, 21205, USA

    Dan Rawlins, Philip Rosenfeld & Thomas Kelly Jr

Authors
  1. Lothar Hennighausen
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  2. Ulrich Siebenlist
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  7. Thomas Kelly Jr
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Hennighausen, L., Siebenlist, U., Danner, D. et al. High-affinity binding site for a specific nuclear protein in the human IgM gene. Nature 314, 289–292 (1985). https://doi.org/10.1038/314289a0

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