Abstract
A bacterial repressor protein blocks transcription of a gene in yeast when its operator is placed in the promoter between the upstream activator sequence and the transcription start point. Putative transcription terminators from yeast installed in the same region of the promoter have a similar effect.
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References
Guarante, L. Cell 36, 799–800 (1984).
West, R., Yocum, R. R. & Ptashne, M. Molec. cell. Biol. 4, 2467–2478 (1984).
Yocum, R. R., Hanley, S., West, R. & Ptashne, M. Molec. cell. Biol. 4, 1985–1998 (1984).
Johnston, M. & Davis, R. W. Molec. cell. Biol. 4, 1440–1448 (1984).
Guarente, L., Yocum, R. R. & Gifford, P. Proc. natl. Acad. Sci. U.S.A. 79, 7410–7414 (1982).
Walker, G. Microbiol. Rev. 48, 60–93 (1984).
Brent, R. in UCLA Symp. molec. cell. Biol. New Ser. Vol. 11 (eds Friedberg, E. C. & Bridges, B. A.) 361–368 (Liss, New York, 1983).
Little, J. W. & Mount, D. W. Cell 29, 11–22 (1982).
Brent, R. & Ptashne, M. Proc. natn. Acad. Sci. U.S.A. 78, 4204–4208 (1981).
Fried, M. & Crothers, D. M. Nucleic Acids Res. 9, 6505–6525 (1981).
Zaret, K. S. & Sherman, F. J. molec. Biol. 177, 107–136 (1984).
Zaret, K. S. & Sherman, F. Cell 18, 563–573 (1982).
Silver, P. A., Keegan, L. P. & Ptashne, M. Proc. nat. Acad. Sci. U.S.A. 81 (in the press).
Hall, M. N., Hereford, L. & Herskowitz, I. Cell 36, 1057–1065 (1984).
Ptashne, M. Nature 308, 753–754 (1984).
Siliciano, P. G. & Tatchell, K. Cell 37, 969–978 (1984).
Khoury, G. & Gruss, P. Cell 33, 313–314 (1983).
Abraham, J. et al. J. molec. Biol. 176, 107–136 (1984);
Maniatis, T., Sambrook, J. & Fritsch, E. F. Molecular Cloning (Cold Spring Harbor Laboratory, New York, 1982).
Sherman, F., Fink, G. R. & Lawrence, C. W. Methods in Yeast Genetics (Cold Spring Harbor Laboratory, New York, 1979).
Groneborn, B. & Messing, J. Nature 272, 375–377 (1978).
Sutcliffe, J. G. & Church, G. M. Nucleic Acids Res. 5, 2313–1329 (1982).
Little, J. W. Proc. natn. Acad. Sci. U.S.A. 81, 1375–1379 (1981).
Laemmli, U. K. Nature 227, 680–685 (1970).
Bennetzen, J. L. & Hall, B. D. J. biol. Chem. 257, 3018–3025 (1982).
Dennis, C. L., Ferguson, J. & Young, E. T. J. biol. Chem. 258, 1165–1171 (1983).
Fraenkel, D. G. in The Molecular Biology of the Yeast Saccharomyces, Vol. 1 (eds Strathern, J. N., Jones, E. W. & Broach, J. R.) 1–37 (Cold Spring Harbor Laboratory, New York, 1983).
Guarente, L. & Ptashne, M. Proc. nat. Acad. Sci. U.S.A. 78, 2199–2203 (1981).
Orr-Weaver, T. L., Szostak, J. W. & Rothstein, R. J. Proc. nat. Acad. U.S.A. 78, 6348–6354 (1981).
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Brent, R., Ptashne, M. A bacterial repressor protein or a yeast transcriptional terminator can block upstream activation of a yeast gene. Nature 312, 612–615 (1984). https://doi.org/10.1038/312612a0
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DOI: https://doi.org/10.1038/312612a0
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