Abstract
The mechanism of leukaemogenk transformation by human T-cell leukaemia/Iymphoma virus (HTLV), a retrovirus implicated in the aetiology of certain adult T-cell leukaemias and lymphomas, is unknown but is conceivably associated with the expression of the cellular analogues of retroviral oncogenes. The HUT-102 cell line1, derived from a cutaneous T-cell lymphoma and infected with HTLV2–4, expresses several cellular oncogenes5. It is unusual among haemopoietic cell lines in that one of these is c-sis, the gene from which the oncogene v-sis of the simian sarcoma virus was derived, and perhaps the gene for platelet-derived growth factor (PDGF)6,7. To explore the possible role of c-sis expression in HTLV-induced disease, we have obtained cDNA clones of c-sis from HUT-102 cells. Here we describe two such clones and report that one of them transforms NIH-3T3 cells. This is the first example of transformation of NIH-3T3 cells by a human onc gene other than c-ras or Blym, as well as the first demonstration of transformation by a human cDNA clone.
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Clarke, M., Westin, E., Schmidt, D. et al. Transformation of NIH 3T3 cells by a human c-sis cDNA clone. Nature 308, 464–467 (1984). https://doi.org/10.1038/308464a0
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DOI: https://doi.org/10.1038/308464a0
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