Abstract
Many transformed cells in culture have been found to express elevated levels of a cellular tumour antigen, termed p531–9. This protein has also been implicated in the regulation of cellular growth10–12. For these reasons experiments were designed to examine the expression of p53 as quiescent cultures of nontrans formed 3T3 fibroblasts were stimulated to reenter the cell cycle. Synchronous populations of cells were obtained by releasing a culture from density-dependent inhibition of growth with the addition of fresh serum13. Steady-state levels of p53 protein and mRNA were measured as a function of time after addition of serum to quiescent cultures and the rate of synthesis of p53 protein was analysed at a number of time points. The results, reported here, demonstrate an increase in the synthesis and steady-state levels of p53 protein and mRNA prior to DNA synthesis in late G1 and suggest a role for p53 in the progression of cells from a growth-arrested state to an actively dividing state.
This is a preview of subscription content, access via your institution
Access options
Subscribe to this journal
Receive 51 print issues and online access
$199.00 per year
only $3.90 per issue
Buy this article
- Purchase on Springer Link
- Instant access to full article PDF
Prices may be subject to local taxes which are calculated during checkout
Similar content being viewed by others
References
Lane, D. P. & Crawford, L. V. Nature 278, 261–263 (1979).
Linzer, D. I. H. & Levine, A. J. Cell 17, 43–52 (1979).
Linzer, D. I. H., Maltzman, W. & Levine, A. J. Virology 98, 308–318 (1979).
DeLeo, A. B. et al. J. Proc. natn. Acad. Sci. 76, 2420–2424 (1979).
Crawford, L. V., Pim, D. C., Gurney, E. G., Goodfellow, P. & Taylor-Papadimitriou, J. Proc. natn. Acad. Sci. U.S.A. 78, 41–45 (1981).
Rotter, V., Witte, O. N., Coffman, R. & Baltimore, D. J. Virol. 36, 547–555 (1980).
Gurney, E. G., Harrison, R. O. & Fenno, J. J. Virol. 34, 752–763 (1980).
Oren, M., Maltzman, W. & Levine, A. J. Molec. cell. Biol. 1, 101–110 (1981).
Oren, M., Reich, N. & Levine, A. J. Molec. Cell. Biol. 2, 443–449
Milner, J. & McCormick, F. Cell Biol. (Int. Rep.) 4, 664–667 (1980).
Milner, J. & Milner, S. Virology 112, 785–788 (1981).
Mercer, W. E., Nelson, D., DeLeo, A. B., Old, L. J. & Baserga, R. Proc. natn. Acad. Sci. U.S.A. 79, 6309–6312 (1982).
Ashihara, T. & Baserga, T. Meth. Enzym. 68, 248–262 (1979).
Burnette, W. N. Analyt. Biochem. 112, 195–203. (1981).
Thomas, P. S. Proc. natn. Acad. Sci. U.S.A. 77, 5201–5205 (1980).
Oren, M. & Levine, A. J. Proc. natn. Acad. Sci, U.S.A. 80, 56–59 (1983).
Cleveland, D. W. et al. Cell 20 95–105 (1980).
Reich, N., Oren, M. & Levine, A. J. Molec. Cell. Biol. 3, 2143–2150 (1983).
Tjian, R., Fey, G. & Graessman, A. Proc. natn. Acad. Sci. U.S.A 75, 1279–1283 (1978).
Harlow, E., Crawford, L. V., Pim, D. C. & Williamson, N. M. J. virol. 39, 861–869 (1981).
Harlow, E., Pim, D. C. & Crawford, L. V. J. Virol. 37, 564–573 (1981).
Sarnow, P., Sullivan, C. A. & Levine, A. J. Virology 120, 510–517 (1982).
Aviv, H. & Leder, P. Proc. natn. Acad. Sci. U.S.A. 69, 1408–1412 (1972).
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Reich, N., Levine, A. Growth regulation of a cellular tumour antigen, p53, in nontransformed cells. Nature 308, 199–201 (1984). https://doi.org/10.1038/308199a0
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1038/308199a0
This article is cited by
-
MicroRNA Cargo in Wharton’s Jelly MSC Small Extracellular Vesicles: Key Functionality to In Vitro Prevention and Treatment of Premature White Matter Injury
Stem Cell Reviews and Reports (2023)
-
Cloning of Hynobius lichenatus (Tohoku hynobiid salamander) p53 and analysis of its expression in response to radiation
BMC Genetics (2020)
-
How does p53 induce apoptosis and how does this relate to p53-mediated tumour suppression?
Cell Death & Differentiation (2018)
-
Obesity and related consequences to ageing
AGE (2016)
-
BAC transgenic mice provide evidence that p53 expression is highly regulated in vivo
Cell Death & Disease (2015)
Comments
By submitting a comment you agree to abide by our Terms and Community Guidelines. If you find something abusive or that does not comply with our terms or guidelines please flag it as inappropriate.
