Abstract
Fish muscle provides a particularly suitable specimen for studying the organization of thick filaments in vertebrate striated muscle because the filaments are arranged with a single orientation on a well ordered hexagonal lattice1. The M-band consists of sets of cross-links, which join the myosin filaments in the middle of the A-band. Previous work1 concluded that the fish muscle M-band had the local symmetry of the dihedral point group 32. We present here a quantitative analysis of transverse sections of the M-band, using general methods2 developed for crystalline layers, to combine various tilted views. The three-dimensional map computed to a resolution of about 70 Å confirms previous findings1; it shows new features of the thick filament structure in the M-band region and provides new information on the use of three-dimensional reconstruction from sectioned biological material. The accompanying paper3 describes a novel technique of three-dimensional reconstruction of the fish M-band from a single view of a slightly oblique plastic section.
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References
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Luther, P., Crowther, R. Three-dimensional reconstruction from tilted sections of fish muscle M-band. Nature 307, 566–568 (1984). https://doi.org/10.1038/307566a0
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DOI: https://doi.org/10.1038/307566a0
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