Abstract
The activity and products of cellular oncogenes can be altered by various processes, such as the nearby integration of a retroviral genome, point mutation within the oncogene coding region, gene amplification, and chromosomal translocation (reviewed in ref. 1). Our work has provided an example of oncogene activation by yet a different process; the integration of an endogenous retrovirus-like DNA element (identified as an intracisternal A particle or IAP genome2) within the coding region of the oncogene c-mos in a mouse plasmacytoma, XRPC 243,4. The rearranged c-mos gene of XRPC24 is actively transcribed and has transforming activity3, suggesting some role for activated c-mos in the progression of the XRPC24 tumour. In this report we describe rearrangement of c-mos in a second mouse plasmacytoma, NSI, and in two hybridomas. In this case, as in XRPC24, c-mos was split by the insertion of a IAP genome. The rearranged c-mos genes (rc-mos) of NSI and XRPC24 differ in three major aspects: (1) The site of IAP integration in c-mos is in codon 30 in NSI but in codon 88 in XRPC24; (2) The orientation of the integrated IAP relative to c-mos is ‘tail-to-head’ in NSI and ‘head-to-head’ in XRPC24; and (3) transcriptional activity of rc-mos in NSI is much lower than in XRPC24. The two latter points suggest a correlation between the orientation of the long terminal repeat (LTR) of IAP relative to c-mos and its activity upon IAP integration.
This is a preview of subscription content, access via your institution
Access options
Subscribe to this journal
Receive 51 print issues and online access
$199.00 per year
only $3.90 per issue
Buy this article
- Purchase on Springer Link
- Instant access to full article PDF
Prices may be subject to local taxes which are calculated during checkout
Similar content being viewed by others
References
Bishop, J. M. A. Rev. Biochem. 52, 301–354 (1983).
Kuff, E. L. et al. Nature 302, 547–548 (1983).
Rechavi, G., Givol, D. & Canaani, E. Nature 300, 607–611 (1982).
Canaani, E. et al. Proc. natn. Acad. Sci. U.S.A. 80, 7118–7122 (1983).
Kohler, G., Howe, S. S. & Milstein, C. Eur. J. Immun. 6, 292–295 (1976).
Parhami-Seren, B., Eshhar, Z. & Mozes, E. Immunology 49, 4–19 (1983).
Van Beveren, C., van Straaten, F., Galleshaw, J. A. & Verma, I. M. Cell 27, 97–108 (1981).
Reddy, E. P. et al. Proc. natn. Acad. Sci. U.S.A. 77, 5234–5238 (1980).
Van Beveren, C. et al. Nature 289, 258–262 (1981).
Gattoni-Celli, S., Hsiao, W. W.-Z. & Weinstein, I. B. Nature (this issue).
Elder, R. T. Loh, E. Y. & Davis, R. W. Proc. natn. Acad. Sci. U.S.A. 80, 2432–2436 (1983).
Scherer, S., Mann, C. & Davis, R. W. Nature 289, 815–819 (1982).
Varmus, H. E. Science 216, 812–820 (1982).
Temin, H. M. Cell 27, 1–3 (1981).
Dansmuir, P., Brorein, W. J., Simon, M. A. & Rubin, G. M. Cell 21, 575–579 (1980).
Will, B. M., Bayev, A. A. & Finnegan, D. J. J. molec. Biol. 153, 897–915 (1981).
Levis, R., Dunsmuir, P. & Rubin, G. M. Cell 21, 581–588 (1980).
Farabaugh, P. J. & Fink, G. R. Nature 287, 352–356 (1980).
Gafner, J. & Philippsen, P. Nature 286, 414–418 (1980).
Kuff, E. L. et al. Proc. natn. Acad. Sci. U.S.A. 80, 1992–1996
Kuff, E. L., Smith, L. A. & Lueders, K. K. Molec. Cell Biol. 1, 216–227 (1981).
Cole, M. D., Ono, M. & Huang, R. C. C. J. Virol. 42, 123–130 (1982).
Maxam, A. M. & Gilbert, W. Meth. Enzym. 65, 499–560 (1980).
Smith, D. R. & Calvo, J. M. Nucleic Acids Res. 8, 2255–2274 (1980).
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Cohen, J., Unger, T., Rechavi, G. et al. Rearrangement of the oncogene c-mos in mouse myeloma NSI and hybridomas. Nature 306, 797–799 (1983). https://doi.org/10.1038/306797a0
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1038/306797a0
This article is cited by
Comments
By submitting a comment you agree to abide by our Terms and Community Guidelines. If you find something abusive or that does not comply with our terms or guidelines please flag it as inappropriate.
