Abstract
Transgenic mice were produced by microinjection of a rearranged, functional immunoglobulin κ gene into fertilized mouse eggs and implantation of the microinjected embryos into foster mothers. Mice that integrated the injected gene were mated and the DNA, RNA and serum κ chains of their offspring were analysed. The data from offspring of three different transgenic mice indicate that the microinjected gene is expressed in the spleen, but not the liver of mice which inherited the injected gene.
This is a preview of subscription content, access via your institution
Access options
Subscribe to this journal
Receive 51 print issues and online access
$199.00 per year
only $3.90 per issue
Buy this article
- Purchase on Springer Link
- Instant access to full article PDF
Prices may be subject to local taxes which are calculated during checkout
Similar content being viewed by others
References
Sakano, H., Huppi, K., Heinrich, G. & Tonegawa, S. Nature 280, 288–294 (1979).
Max, E., Seidman, J. & Leder, P. Proc. natn. Acad. Sci. U.S.A. 76, 3450–3454 (1979).
Brinster, R. L., Chen, H. Y. & Trumbauer, M. E. Science 211, 396–398 (1981).
Brinster, R. L. et al. Cell 27, 223–231 (1981).
Palmiter, R. D., Chen, H. Y. & Brinster, R. L. Cell 29, 701–710 (1982).
Palmiter, R. D. et al. Nature 300, 611–615 (1982).
Walfield, A. M., Storb, U., Selsing, E. & Zentgraf, H. Nucleic Acids Res. 8, 4689–4707 (1980).
Walfield, A., Selsing, E., Arp, B. & Storb, U. Nucleic Acids Res. 9, 1101–1109 (1981).
Kabat, E. A., Wu, T. T. & Bilofsky, H. in Sequences of Immunoglobulin Chains, NIH Publ. No. 80-2008 (1979).
Potter, M. Adv. Immun. 25, 141–207 (1977).
Selsing, E. & Storb, U. Nucleic Acids Res. 9, 5725–5735 (1981).
Storb, U., Arp, B. & Wilson, R. Nucleic Acids Res. 8, 4681–4687 (1980).
Storb, U., Near, R., Putnam, D. & Clagett, J. Molec. Immun. 17, 947–957 (1980).
Storb, U., Hager, L., Wilson, R. & Putnam, D. Biochemistry 16, 5432–5438 (1977).
O'Farrell, P. H. J. biol. Chem. 250, 4007–4021 (1975).
Wagner, T. E. et al. Proc. natn. Acad. Sci. U.S.A. 78, 6376–6380 (1981).
Constantini, F. & Lacy, E. Nature 294, 92–94 (1981).
Wagner, E. R., Stewart, T. A. & Mintz, B. Proc. natn. Acad. Sci. U.S.A. 78, 5016–5020 (1981).
Storb, U., Wilson, R., Selsing, E. & Walfield, A. Biochemistry 20, 990–996 (1981).
Storb, U. & Arp, B. Proc. natn. Acad. Sci. U.S.A. (in the press).
Perry, R. P. et al. Proc. natn. Acad. Sci. U.S.A. 77, 1937–1941 (1980).
Hieter, P. A., Max, E. E., Seidman, J. G., Maizel, J. V. Jr & Leder, P. Cell 22, 197–207 (1980).
Parslow, T. G. & Granner, D. K. Nature 299, 449–451 (1982).
Boss, M. A. Nature 303, 281–282 (1982).
Queen, C. & Baltimore, D. Cell 33, 741–748 (1983).
Emorine, L., Kuehl, M., Weir, L., Leder, P. & Max, E. Nature 304, 447–449 (1983).
Gillies, S. D., Morrison, S. L., Oi, V. T. & Tonegawa, S. Cell 33, 717–728 (1983).
Banerji, J., Olson, L. & Schaffner, W. Cell 33, 729–740 (1983).
Mercola, M., Wang, X., Olsen, J. & Calame, K. Science 221, 663–665 (1983).
Perry, R. P., Kelley, D. E., Coleclough, C. & Kearney, J. F. Proc. natn. Acad. Sci. U.S.A. 78, 247–251 (1981).
Southern, E. M. J. molec. Biol. 98, 503–517 (1975).
McMaster, G. K. & Carmichael, G. G. Proc. natn. Acad. Sci. U.S.A. 64, 4835–4839 (1977).
Thomas, P. S. Proc. natn. Acad. Sci. U.S.A. 77, 5201–5205 (1981).
Denhardt, D. Biochem. biophys. Res. Commun. 23, 641–652 (1966).
Merrill, C. R., Goldman, D., Sedman, S. A. & Ebert, M. H. Science 211, 1437–1438 (1981).
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Brinster, R., Ritchie, K., Hammer, R. et al. Expression of a microinjected immunoglobulin gene in the spleen of transgenic mice. Nature 306, 332–336 (1983). https://doi.org/10.1038/306332a0
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1038/306332a0
This article is cited by
-
Dual regulation of proliferation and apoptosis: c-myc in bitransgenic murine mammary tumor models
Oncogene (2000)
-
Transgenic mice expressing a chimaeric anti-E. coli immunoglobulin α heavy chain gene
Transgenic Research (1994)
-
Gene transfer efficiency during gestation and the influence of co-transfer of non-manipulated embryos on production of transgenic mice
Transgenic Research (1994)
-
Transgenic regulation in laboratory animals
Experientia (1991)
Comments
By submitting a comment you agree to abide by our Terms and Community Guidelines. If you find something abusive or that does not comply with our terms or guidelines please flag it as inappropriate.