Abstract
The centromere region of yeast chromosome III has been investigated by altering it in vivo. Deleting the functional centromere (CEN3) sequence leads to extreme instability of the resulting acentric chromosome. Inversion of CEN3, or its replacement by chromosome XI centromere DNA (CEN11) has no measurable effect on the mitotic and meiotic behaviour of chromosome III, suggesting that yeast centromeres are not chromosome-specific, and are fully functional in either orientation.
This is a preview of subscription content, access via your institution
Access options
Subscribe to this journal
Receive 51 print issues and online access
$199.00 per year
only $3.90 per issue
Buy this article
- Purchase on Springer Link
- Instant access to full article PDF
Prices may be subject to local taxes which are calculated during checkout
Similar content being viewed by others
References
Clarke, L. & Carbon, J. Nature 287, 504–509 (1980).
Fitzgerald-Hayes, M., Buhler, J.-M., Cooper, T. & Carbon, J. Molec. Cell Biol. 2, 82–87 (1982).
Stinchcomb, D. T., Mann, C. & Davis, R. W. J. molec. Biol. 158, 157–179 (1982).
Panzeri, L. & Philippsen, P. EMBO J. 1, 1605–1611 (1982).
Fitzgerald-Hayes, M., Clarke, L. & Carbon, J. Cell 29, 235–244 (1982).
Rothstein, R. J. Meth. Enzym. 101, 202–211 (1983).
Stinchcomb, D. T., Struhl, K. & Davis, R. W. Nature 282, 39–43 (1979).
Barry, E. G. Genetics 71, 53–62 (1972).
Perkins, D. D. & Barry, E. G. Adv. Genet. 19, 133–285 (1977).
Southern, E. M. J. molec. Biol. 98, 503–517 (1975).
Hartwell, L. H., Dutcher, S. K., Wood, J. S. & Garvik, B. Rec. Adv. Yeast molec. Biol. 1 (1982).
Johnson, J. R. Genet. Res. 18, 179–184 (1971).
Skogerson, L., McLaughlin, C. & Wakatama, E. J. Bact. 116, 818–822 (1973).
Kasha, K. J. Proc. 12th int. Congr. Genet. 1, (abstr.), 172 (1968).
Mortimer, R. K. & Hawthorne, D. C. Meth. Cell Biol. 11, 221–233 (1975).
Mortimer, R. K. & Schild, D. Microbiol. Rev. 44, 519–571 (1980).
Dutcher, S. K. & Hartwell, L. H. Genetics 100, 175–184 (1982).
Bach, M., Lacroute, F. & Botstein, D. Proc. natn. Acad. Sci. U.S.A. 76, 386–390 (1979).
Hinnen, A., Hicks, J. B. & Fink, G. B. Proc. natn. Acad. Sci. U.S.A. 75, 1929–1933 (1978).
Orr-Weaver, T. L., Szostak, J. W. & Rothstein, R. J. Proc. natn. Acad. Sci. U.S.A. 78, 6354–6358 (1981).
Tschumper, G. T. & Carbon, J. Gene 23, 221–232 (1983).
Reed, S. I. Gene 20, 255–265 (1982).
Rigby, P. W., Dieckmann, M., Rhodes, C. & Berg, P. J. molec. Biol. 113, 237–251 (1977).
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Clarke, L., Carbon, J. Genomic substitutions of centromeres in Saccharomyces cerevisiae. Nature 305, 23–28 (1983). https://doi.org/10.1038/305023a0
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1038/305023a0
This article is cited by
-
The molecular basis for centromere identity and function
Nature Reviews Molecular Cell Biology (2016)
-
Couples, pairs, and clusters: mechanisms and implications of centromere associations in meiosis
Chromosoma (2014)
-
Centromeres: unique chromatin structures that drive chromosome segregation
Nature Reviews Molecular Cell Biology (2011)
-
A centromeric DNA sequence colocalized with a centromere-specific histone H3 in tobacco
Chromosoma (2009)
-
Unstable transmission of rice chromosomes without functional centromeric repeats in asexual propagation
Chromosome Research (2009)
Comments
By submitting a comment you agree to abide by our Terms and Community Guidelines. If you find something abusive or that does not comply with our terms or guidelines please flag it as inappropriate.
