Abstract
Template-active regions of chromatin are structurally distinct from nontranscribing segments of the genome. Recently, it was suggested that the conformation of active genes which renders them sensitive to DNase I may be maintained even in fixed mitotic chromosomes. We have developed a technique of mitotic cell fixation and DNase I-directed nick-translation which distinguishes between active and inactive X chromosomes. We report here that Gerbillus gerbillus (rodent) female cells contain easily identified composite X chromosomes each of which includes the original X chromosome flanked by two characteristic autosomal segments. After nick-translation the active X chromosome in each cell is labelled specifically in both the autosomal and X-chromosomal regions. The inactive X chromosome is labelled only in the autosomal regions and in a small early replicating band within the late replicating ‘original X’ chromosome. Our technique opens the possibility of following the kinetics of X-chromosome inactivation and reactivation during embryogenesis, studying active genes in the inactive X chromosome and mapping tissue-specific gene clusters.
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References
Weintraub, H. & Groundine, M. Science 193, 848–856 (1976).
Bellard, M., Gannon, F. & Chambon, P. Cold Spring Harb. Symp. quant. Biol. 42, 729–791 (1977).
Gottesfeld, J. & Partington, G. Cell 12, 953–962 (1977).
Garel, A. & Axel, R. Proc. natn. Acad. Sci. U.S.A. 73, 3966–3970 (1976).
Panet, A. & Cedar, H. Cell 11, 933–940 (1977).
Garel, A., Zolan, M. & Axel, R. Proc. natn. Acad. Sci. U.S.A. 74, 4867–4871 (1977).
Stalder, J., Groundine, M., Dodgson, J.B., Engel, J.D. & Weintraub, H. Cell 19, 973–980 (1980).
Gazit, B., Cedar, H., Lehrer, I. & Voss, R. Science 217, 648–650 (1982).
Wahrman, J., Richler, C., Neufeld, E. & Friedmann, A. Cytogenet. Cell Genet. 35, 161–181 (1983).
Zakharov, A. F. & Egolina, N. A. Chromosoma 38, 341–365 (1972).
Latt, S. A. Proc. natn. Acad. Sci. U.S.A. 70, 3395–3399 (1973).
Sarto, G. E., Therman, E. & Patau,, K. Clin. Genet. 6, 289–293 (1974).
McKusick, V. A. & Chase, G. A. A. Rev. Genet. 7, 435–473 (1973).
Shapiro, L. J., Mohandas, T., Weiss, R. & Romeo, G. Science 204, 1224–1226 (1979).
Müller, C. R., Westerveld, A., Beate Migl, B., Franke, W. & Roppers, H. H. Hum. Genet. 54, 201–204 (1980).
Willard, H. F. & Latt, S. A. Am. J. hum. Genet. 28, 213–227 (1976).
Therman, E., Sarto, G.E., Distèche, C. & Denniston, C. Chromosoma 59, 137–145 (1976).
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Kerem, BS., Goitein, R., Richler, C. et al. In situ nick-translation distinguishes between active and inactive X chromosomes. Nature 304, 88–90 (1983). https://doi.org/10.1038/304088a0
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DOI: https://doi.org/10.1038/304088a0
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