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Hepatoma secretory proteins migrate from rough endoplasmic reticulum to Golgi at characteristic rates

Nature volume 304, pages 80–83 (1983)Cite this article

Abstract

In eukaryotic cells, secretory proteins and glycoproteins migrate from the rough endoplasmic reticulum, their site of synthesis, through Golgi vesicles before being released from the cell1–9. Cellular and viral integral plasma membrane glycoproteins are co-translationally inserted into the rough endoplasmic reticulum membrane and follow a similar pathway to the cell surface2,3,10–15. Previous studies using endoglycosidase H (Endo H) suggested that in rat hepatoma cells the vesicular stomatitis virus (VSV) G protein, albumin and transferrin migrate from the rough endoplasmic reticulum to the Golgi apparatus at different rates16. Here we show directly that in human hepatoma HepG2 cells, five secreted proteins mature from the rough endoplasmic reticulum to Golgi vesicles at characteristic rates which differ at least threefold. The results are incompatible with bulk-phase movement of the luminal contents of the endoplasmic reticulum, and suggest that there is a membrane-bound receptor that selectively mediates the transport of secretory proteins from the rough endoplasmic reticulum to the Golgi.

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Author notes

  1. Martin Snider

    Present address: Department of Embryology, Carnegie Institute of Washington, 115 West University Parkway, Baltimore, Maryland, 21210, USA

  2. Ger J. A. M. Strous

    Present address: Laboratory of Histology and Cell Biology, Nic Beetsstraat 22, 3511 HG, Utrecht, The Netherlands

Authors and Affiliations

  1. Department of Biology, Massachusetts Institute of Technology, Cambridge, Massachusetts, 02139, USA

    Harvey F. Lodish, Nancy Kong, Martin Snider & Ger J. A. M. Strous

  2. Department of Embryology, Carnegie Institute of Washington, 115 West University Parkway, Baltimore, Maryland, 21210, USA

    Ger J. A. M. Strous

Authors
  1. Harvey F. Lodish
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  2. Nancy Kong
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  3. Martin Snider
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  4. Ger J. A. M. Strous
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Lodish, H., Kong, N., Snider, M. et al. Hepatoma secretory proteins migrate from rough endoplasmic reticulum to Golgi at characteristic rates. Nature 304, 80–83 (1983). https://doi.org/10.1038/304080a0

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