Abstract
Specific genes can be introduced into cultured mammalian cells by DNA transfer1, sometimes to be stably integrated and expressed2,3. Because interferon inhibits the transformation initiated by DNA viruses4–8, we wondered if it might also affect transformation induced by recombinant plasmids and cellular genes. We show here that in mouse L cells interferon treatment prevents stable integration and expression of the transfected plasmids containing the cloned herpesvirus thymidine kinase (tk) gene, or the dihydrofolate reductase (dhfr) gene from hamster ovary cells9. In contrast, interferon does not prevent transient expression of the tk gene in unintegrated form.
This is a preview of subscription content, access via your institution
Access options
Subscribe to this journal
Receive 51 print issues and online access
$199.00 per year
only $3.90 per issue
Buy this article
- Purchase on Springer Link
- Instant access to full article PDF
Prices may be subject to local taxes which are calculated during checkout
Similar content being viewed by others
References
Pellicer, A. et al. Science 209, 1414–1422 (1980).
Wigler, M. et al. Cell 11, 223–232 (1977).
Wigler, M. et al. Proc. natn. Acad. Sci. U.S.A. 76, 1373–1376 (1979).
Todaro, G. J. & Baron, S. Proc. natn. Acad. Sci. U.S.A. 54, 752–756 (1965).
Todaro, G. J. & Green, H. Proc. natn. Acad. Sci. U.S.A. 55, 302–308 (1966).
Taylor-Papadimitriou, J. & Stoker, M. Nature new Biol. 230, 114–117 (1971).
Rapp, F. & Nishiyama, Y. J. Virol. 33, 543–546 (1980).
Turek, L. P. et al. UCLA Symp. molec. cell. Biol. 25, 181–191 (1982).
Flintoff, W. F., Davidson, S. V. & Siminovitch, L. Somatic Cell Genet. 2, 245–261 (1976).
Dubois, M. F., Vignal, M. & Chany, C. C. r. hebd. Séanc. Acad. Sci., Paris 295, 195–197 (1982).
Colbere-Garapin, F., Chousterman, S., Horodniceanu, F., Kourilski, P. & Garapin, A. C. Proc. natn. Acad. Sci. U.S.A. 76, 3755–3759 (1979).
Graham, F. L. & Van der Eb, A. J. Virology 52, 456–467 (1973).
Sandri-Goldin, R. M., Goldin, A. L., Levine, M. & Glorioso, J. Molec. cell. Biol. 1, 743–752 (1981).
Wigler, M. et al. Proc. natn. Acad. Sci. U.S.A. 77, 3567–3570 (1980).
Chang, L. J. A. et al. Proc. natn. Acad. Sci. U.S.A. 79, 146–150 (1982).
Linsley, P. S. & Siminovitch, L. Molec. cell. Biol. 2, 593–597 (1982).
Loyter, A., Scangos, G. A. & Ruddle, F. H. Proc. natn. Acad. Sci. U.S.A. 79, 422–426 (1982).
Capecchi, M. R. Cell 22, 479–488 (1980).
Dussaix, E., Grégoire, A., Chany, C., Thang, D. C. & Thang, M. N. J. gen. Virol. 64, 285–290 (1983).
Szybalska, E. H. & Szybalski, W. Proc. natn. Acad. Sci. U.S.A. 48, 2026–2034 (1962).
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Dubois, MF., Vignal, M., Cunff, M. et al. Interferon inhibits transformation of mouse cells by exogenous cellular or viral genes. Nature 303, 433–435 (1983). https://doi.org/10.1038/303433a0
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1038/303433a0
This article is cited by
-
Antitumor therapy based on the use of an immune modulator, arginine butyrate and interferon
Medical Oncology and Tumor Pharmacotherapy (1984)
Comments
By submitting a comment you agree to abide by our Terms and Community Guidelines. If you find something abusive or that does not comply with our terms or guidelines please flag it as inappropriate.