Abstract
Two-dimensional crystals are formed from macromolecules bound on the surface of a lipid monolayer. A ligand linked to the lipid orientates the binding, and lateral diffusion of the lipids facilitates crystallization. The crystals are suitable for structural analysis by image processing of electron micrographs. An example is the formation of ordered arrays of antibodies on a monolayer of a lipid hapten, and subsequent decoration of these arrays with the first component of complement. Image processing indicates the arrangement of antibodies and the site of complement binding. This approach should be widely applicable to molecular complexes, such as those in replication, protein synthesis, hormone–receptor interaction and metabolic processes.
This is a preview of subscription content, access via your institution
Access options
Subscribe to this journal
Receive 51 print issues and online access
$199.00 per year
only $3.90 per issue
Buy this article
- Purchase on Springer Link
- Instant access to full article PDF
Prices may be subject to local taxes which are calculated during checkout
Similar content being viewed by others
References
DeRosier, D. J. & Klug, A. Nature 217, 130–134 (1968).
Crowther, R. A. & Klug, A. A. Rev. Biochem. 44, 161–182 (1975).
Unwin, P. N. T. & Henderson, R. J. molec. Biol. 94, 425–440 (1975).
Renyi, A. Trans. math. statist. Problems 4, 203–244 (1963).
Feder, J. & Giaever, I. J. Colloid Interface Sci. 78, 144–154 (1980).
Kornberg, R. D. & McConnell, H. M. Proc. natn. Acad. Sci. U.S.A. 68, 2564–2568 (1971).
Small, D. M. J. Lipid Res. 8, 551–557 (1967).
Weis, R. M., Balakrishnan, K., Smith, B. A. & McConnell, H. M. J. biol. Chem. 257, 6440–6445 (1982).
Fromherz, P. Nature 231, 267–268 (1971).
Sarma, V. R., Silverton, E. W., Davies, D. R. & Terry, N. D. J. biol. Chem. 246, 3753–3759 (1971).
Labaw, L. W. & Davies, D. R. J. ultrastruct. Res. 40, 349–365 (1972).
DeRosier, D. J. & Moore, P. B. J. molec. Biol. 52, 355–369 (1970).
Amos, L. A., Henderson, R. & Unwin, P. N. T. Prog. Biophys. molec. Biol. (in the press).
Müller-Eberhard, H. J. A. Rev. Biochem. 44, 697–724 (1975).
Reid, K. B. M. & Porter, R. R. A. Rev. Biochem. 50, 433–464 (1981).
Shelton, E., Yonemasu, K. & Stroud, R. M. Proc. natn. Acad. Sci. U.S.A. 69, 65–68 (1972).
Knobel, H. R., Wiliger, W. & Isliker, H. Eur. J. Immun. 5, 78–82 (1975).
Reed, K. B. M. & Porter, R. R. Biochem. J. 155, 19–23 (1976).
Reidler, J. et al. J. molec. Biol. (in the press).
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Uzgiris, E., Kornberg, R. Two-dimensional crystallization technique for imaging macromolecules, with application to antigen–antibody–complement complexes. Nature 301, 125–129 (1983). https://doi.org/10.1038/301125a0
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1038/301125a0
This article is cited by
-
Immunoactive two-dimensional self-assembly of monoclonal antibodies in aqueous solution revealed by atomic force microscopy
Nature Materials (2014)
-
An autobiographic conversation with Roger D Kornberg on his work on transcription regulation
Cell Death & Differentiation (2007)
-
The molecular basis of eucaryotic transcription
Cell Death & Differentiation (2007)
-
Specific binding of avidin to biotin containing lipid lamella surfaces studied with monolayers and liposomes
European Biophysics Journal (1995)
-
Epitaxial growth of protein crystals on lipid layers
Nature Structural Biology (1994)
Comments
By submitting a comment you agree to abide by our Terms and Community Guidelines. If you find something abusive or that does not comply with our terms or guidelines please flag it as inappropriate.