Abstract
Annealing of single-stranded (ss) DNA rings of complementary base sequence gives rise to the so-called form V DNA which is characterized by a linking number of zero1. The similarity of the circular dichroism spectrum of form V DNA1 with that of the 1:1 mixture of the low salt (B-type) and the high salt (Z-type) form of poly(dG-dC) ⋅ poly(dG-dC)2,3 suggests that form V DNA might contain left-handed double-helical DNA of the Z-type. The discovery that the left-handed Z-form of poly(dG-dC) ṁ poly(dG-dC) induces a strong immune response4,5 has provided a novel means of searching for DNA of this particular conformation6. We therefore used antisera and monoclonal antibodies to Z-DNA to determine whether form V DNA contains stretches of left-handed double-helical conformation. We report here that form V DNA competes in the binding of antibodies to Z-form poly(dG-dC) · poly(dG-dC). The competition by form V DNA is similar to that of (dG-dC)5·(dG-dC)5. Thus, DNA of natural base sequence, when forced to adopt left-handed conformations by topological restrictions, is recognized by antibodies against Z-DNA
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References
Stettler, V. H., Weber, H., Koller, Th. & Weissmann, Ch. J. molec. Biol. 131, 21–40 (1979).
Pohl, F. M. & Jovin, T. M. J. molec. Biol. 67, 375–396 (1972).
Pohl, F. M. Nature 260, 365–366 (1976).
Lafer, E. M., Möller, A., Nordheim, A., Stollar, B. D. & Rich, A. Proc. natn. Acad. Sci. U.S.A. 78, 3546–3550 (1981).
Malfoy, B. & Leng, M. FEBS Lett. 132, 45–48 (1981).
Nordheim, A. et al. Nature 294, 417–422 (1981).
Thamann, T. J., Lord, R. C., Wang, A. H.-J. & Rich, A. Nucleic Acids Res. 9, 5443–5457 (1981).
Patel, D. J., Canuel, L. L. & Pohl, F. M. Proc. natn. Acad. Sci. U.S.A. 76, 2508–2511 (1979).
Wang, A. H.-J. et al. Nature 282, 680–686 (1979).
Drew, H., Takano, T., Tanaka, S., Itakura, K. & Dickerson, R. E. Nature 286, 567–573 (1980).
Klysik, J., Stirdivant, S. M., Larson, J. E., Hart, P. A. & Wells, R. D. Nature 290, 672–677 (1981).
Pohl, F. M. Eur. J. Biochem. 42, 495–504 (1974).
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Pohl, F., Thomae, R. & DiCapua, E. Antibodies to Z-DNA interact with form V DNA. Nature 300, 545–546 (1982). https://doi.org/10.1038/300545a0
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DOI: https://doi.org/10.1038/300545a0
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